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Intérêt de l'étude moléculaire du gène de la protéine M pour la caractérisation du métapneumovirus humain et des virus influenza équins

Abstract : The human metapneumovirus and equine influenza viruses, H3N8 subtypes, belong to the Paramyxoviridae and Orthomyxoviridae Family, respectively. The reported work evaluates the relevance of the M and M1 genes, coding for matrix protein, 1/ in detecting viral RNA by molecular genetic tools 2/ for phylogenetic purposes. For the hMPV, molecular genetic tools have been developed against the M gene because a study, the first detection of hMPV in France, was carried out using RT-PCR targeting the N gene. Since the tests being developed against the N gene did not allow the identification of all group of hMPV, a rRT-PCR following by a nested PCR were set up in the M gene and used to perform a phylogenetic characterization of hMPV strains circulating in Normandy between 2002 and 2005. All groups and subgroups of hMPV (A1, A2, B1 and B2) were detected. Subsequently, these methods were adapted to a multiplex detection tool, also including human respiratory syncytial virus and influenza virus A and B. Considering EIV, a real-time RT-PCR was developed in the M1 gene, which was used to detect EIV circulating in France between 2005 and 2010. Phylogenic analyses were also conducted even not in the M1 gene, which was insufficiently variable. H3 gene, encoding the major EIV surface protein, was used to determine the different variants. This study revealed for the first time in France the presence of an EIV American lineage, cluster Florida. Molecular genetic tools developed in M and M1 genes thus allowed the detection of both viruses. While Paramyxoviridae M gene only seems suitable for studying viral variability, H3 gene remains the most appropriate for EIV for this purpose.
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https://hal-normandie-univ.archives-ouvertes.fr/tel-02946356
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Submitted on : Wednesday, September 23, 2020 - 9:44:18 AM
Last modification on : Wednesday, September 23, 2020 - 9:44:18 AM

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  • HAL Id : tel-02946356, version 1

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Loïc Legrand. Intérêt de l'étude moléculaire du gène de la protéine M pour la caractérisation du métapneumovirus humain et des virus influenza équins. Médecine vétérinaire et santé animale. université Caen, 2011. Français. ⟨tel-02946356⟩

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