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Apports des outils de génétique moléculaire à la connaissance de deux infections virales du cheval : herpèsvirus équin 1 et artérite virale équine

Abstract : Many viruses are responsible of equine pathologies and may involve both outbreaks and trade limitations. Among them, equid herpesvirus -1 (EHV-1) and equine arteritis virus (EAV) are monitored closely. Methods of detection and molecular characterisation were developed. We investigated the relationships between the different clinical expressions of EHV-1 infection and genotype of the strains being present in France. We could confirm EHV-1 being both a major abortive agent and also responsible of either sporadic or epidemic neurological diseases. Determining by SNP-PCR the presence/absence of mutation A/G2254 in ORF 30, coding for DNA polymerase, allowed to precise that non-neuropathogenic strains could be detected during paralytic forms, and conversely, neuropathogenic strains could be detected during abortive forms of the disease. This suggests that other factors related to horse and environment also are interfering with the clinical expression of the syndrome. Characterisation of the French strains of EAV, by phylogenetic analyses of ORF 2a-7, allowed demonstrating the emergence in 2003 of a North American strain. The outbreak of equine viral arteritis being described in this paper, revealed an European strain from subgroup 2 (highly virulent) to be responsible of one abortion and death of seven horses. Perspectives are based on the complete sequencing of EHV-1 genome as well as phylogenetic study of other EAV strains in order to determine the origin of the outbreak.
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https://hal-normandie-univ.archives-ouvertes.fr/tel-02946345
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Submitted on : Wednesday, September 23, 2020 - 9:37:00 AM
Last modification on : Wednesday, October 14, 2020 - 4:13:05 AM

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  • HAL Id : tel-02946345, version 1

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Stéphane Pronost. Apports des outils de génétique moléculaire à la connaissance de deux infections virales du cheval : herpèsvirus équin 1 et artérite virale équine. Médecine vétérinaire et santé animale. université caen, 2010. Français. ⟨tel-02946345⟩

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