Skip to Main content Skip to Navigation
Theses

Caractérisation biochimique et microscopique du piège extracellulaire de racine et des exsudats racinaires de trois essences ligneuses sahéliennes : Balanites aegyptiaca D. , Acacia tortilis subsp. raddiana S. et Tamarindus indica L.

Abstract : The root cap is primordial for seedling growth and supports root apical meristem integrity. The root cap releases root border cells (RBCs) that surround the root tip and ensure seedling protection against numerous stresses. RBCs and their associated mucilage form the root extracellular trap (RET). Here, RET and root exudate characterization of three Sahelian woody seedlings are performed. In contrast to B. aegyptiaca and A. raddiana which thrive in semi-arid areas, T. indica is more sensitive to drought. B. aegyptiaca, A. raddiana and T. indica seedlings were sub-cultured in vitro. RBC morphologies and RET organization were determined using microscopic approaches. The polysaccharide composition and arabinogalactan protein (AGP) content were determined by biochemical approaches in the RET and the root exudates. Moreover, the effect of root exudates on the growth of Azospirillum brasilense a plant benefical bacteria has been performed. While B. aegyptiaca produces only border cell (BC) type, the two Fabaceae seedlings release both BCs and border-like cells (BLCs). BCs are enclosed in a dense mucilage enriched in cell wall polymers. Compared to T. indica, RET and root exudates of B. aegyptiaca and A. raddiana include more abundant AGPs. In this context, AGPs could contribute to woody seedling survival. This work opens new research perspectives regarding involvement of RET in plant survival to aridity.
Complete list of metadatas

Cited literature [176 references]  Display  Hide  Download

https://hal-normandie-univ.archives-ouvertes.fr/tel-02303890
Contributor : Arnaud Lehner <>
Submitted on : Wednesday, October 2, 2019 - 4:49:30 PM
Last modification on : Wednesday, October 9, 2019 - 1:34:50 AM

Identifiers

  • HAL Id : tel-02303890, version 1

Citation

Alexis Carreras. Caractérisation biochimique et microscopique du piège extracellulaire de racine et des exsudats racinaires de trois essences ligneuses sahéliennes : Balanites aegyptiaca D. , Acacia tortilis subsp. raddiana S. et Tamarindus indica L.. Amélioration des plantes. Université de Rouen Normandie, 2018. Français. ⟨tel-02303890⟩

Share

Metrics

Record views

42

Files downloads

9