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Etude de la morphogenèse et de la voie de N-glycosylation chez la diatomée P. tricornutum

Abstract : N-glycosylation is a major co- and post-translational event of the protein biosynthesis in Eukaryotes. Actually, little information regarding this processing is available in microalgae. In this work, we continued the exploration of the N-glycosylation pathway in the model diatom P. tricornutum by focusing especially on the characterization of three putative α1,3-fucosyltransferases. Our analyzes question the annotation for two of the genes encoding for these fucosyltransferases and propose new ones. In order to achieve the subcellular localization of these glycosyltransferases, we used an over-expressed fusion tag proteins strategy combined together with microscopy approaches. Observations performed by confocal microscopy and TEM allowed us to demonstrate the Golgi subcellular localization of these enzymes. This represent the first subcellular localization of glycosyltransferases in microalgae. P. tricornutum possess three different morphotypes named oval, fusiform and triradiate. The transition from one morphotype to another strongly depends on the environmental conditions. However, little information is available regarding the mechanisms and physiological significance of this morphogenesis. In order to provide new knowledge related to the morphogenesis in P. tricornutm, we conducted a comparative transcriptome analysis of the three morphotypes by an RNA-Seq approach. Besides expanding our knowledge about P. tricornutum morphogenesis, data also helped to identify new reference genes for qRT-PCR analysis
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  • HAL Id : tel-02301887, version 1

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Clément Ovide. Etude de la morphogenèse et de la voie de N-glycosylation chez la diatomée P. tricornutum. Biochimie, Biologie Moléculaire. Université de Rouen Normandie, 2016. Français. ⟨tel-02301887⟩

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