.. .. I-2-l'appareil-de-golgi,

, Techniques développées pour étudier l'appareil

. Ii-biogenèse and .. .. De-golgi, 47 II-1-1-Synthèse de novo à partir du réticulum endoplasmique

.. .. Ii-2-le-réseau-trans-golgien, 50 II-3-Comment est conservée l'intégrité d'un empilement golgien ?

.. .. Ii-4-le-système-endomembranaire-golgien, 52 II-4-2-Transports antérograde et rétrograde entre le RE et les empilements golgiens

. .. , 58 III-2-1-Longueur et nature du domaine transmembranaire des protéines

, 62 III-3-2-Complexes enzymatiques impliqués dans la synthèse du RG-I, III-3-Les enzymes de synthèse fonctionnent

, III-4-Les enzymes de synthèse sont spécifiques d'un compartiment golgien

P. .. De-these,

. Chapitre, . Materiel, and . .. Methodes, , vol.87

. Partie-b-:-creation, . Des, and . .. De-fusion, 88 I-Construction des protéines de fusion avec la Green Fluorescent Protein

, 91 I-1-4-Sous-clonage dans le vecteur d'expression pBLTI-121

G. .. Le-système, 93 I-2-3-Insertion du gène d

, Digestion contrôle Asc1/Not1

.. .. Séquençage, 95 I-2-6-Insertion dans le vecteur d'expression pMDC : la réaction de LR clonase, p.95

, 96 II-1-1-Principe du USER? (uracil-specific excision reagent), II-Construction des protéines de fusion pour la Bimolecular Fluorescence

. Partie-c-:-expression, . Des, and . .. De-fusion, 99 I-1-1-Préparation d'agrobactéries chiomiocompétentes

, 100 I-2-1-Transformation des agrobactéries chiomiocompétentes

, I-2-2-Transformation des agrobactéries électrocompétentes

, 101 II-1-Transformation transitoire des cellules épidermiques de feuilles de tabac Nicotiana tabacum

. Partie-d-:-localisation, . Des, and . .. De-fusion, 102 I-1-Principe de la microscopie confocale à balayage laser (MCBL)

. .. Ii-microscopie-electronique-À-transmission, 104 II-2-2 Cryofixation des échantillons sous haute pression

, 107 II-4-2-Immunomarquage des protéines fusionnées à la GFP

, Coloration et observation des coupes ultrafines

, 109 II-6-2-Distribution golgienne des particules d'or : Test du RLI

P. .. Bifc), 111 I-Principe de la Bimolecular Fluorescence Complementation (BiFC)

. .. Chapitre-iii:-resultats,

, En tant que préambule à l'ensemble de ces travaux, nous avons écrit et publié une

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