Behavior of B- and Z-DNA Crystals under High Hydrostatic Pressure

Thierry Prangé (1) , Nathalie Colloc’h (2) , Anne-Claire Dhaussy (3) , Marc Lecouvey (4) , Evelyne Migianu-Griffoni (4) , Eric Girard (5)

1 CiTCoM - UMR 8038 - Cibles Thérapeutiques et conception de médicaments
2 ISTCT - Imagerie et Stratégies Thérapeutiques pour les Cancers et Tissus cérébraux
3 CRISMAT - Laboratoire de cristallographie et sciences des matériaux
4 CSPBAT - Chimie, Structures et Propriétés de Biomatériaux et d'Agents Thérapeutiques
5 IBS - UMR 5075 - Institut de biologie structurale

Single crystals of B-DNA and Z-DNA oligomers were analyzed under high hydrostatic pressure and their behavior compared to the A-DNA crystals already known. The amplitude of the base compression, when compared to the A-form of DNA (0.13 Å/GPa), was higher for the Z DNA (0.32 Å/GPa) and was the highest for the B-DNA (0.42 Å/GPa). The B-DNA crystal degraded rapidly around 400-500 MPa while the Z-structure was more resistant, up to 1.2 GPa.

Keywords: high pressure; B-DNA dodecamer; Z-DNA hexamer; crystal; X-ray diffraction; DNA

Domains: Life Sciences [q-bio]

Thierry Prangé, Nathalie Colloc’h, Anne-Claire Dhaussy, Marc Lecouvey, Evelyne Migianu-Griffoni, et al.. Behavior of B- and Z-DNA Crystals under High Hydrostatic Pressure. Crystals, 2022, 12, ⟨10.3390/cryst12060871⟩. ⟨hal-03781069⟩