Contributions to Arabinogalactan Protein Analysis

Romain Castilleux (1) , Marc Ropitaux (1) , Youssef Manasfi (1) , Sophie Bernard (1) , Maïté Vicré-Gibouin (1) , Azeddine Driouich (1)

1 Glyco-MEV - Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale

Arabinogalactan proteins (AGPs) are important plant proteoglycans involved in many development processes. In roots, AGPs occur in the cell wall of root cells and root cap–derived cells as well as in the secreted mucilage. Detection, localization , and quantification techniques are therefore essential to unravel the AGP diversity of structures and functions. This chapter details root-adapted immunocytochemical methods using monoclonal antibodies, and a collection of biochemical analysis protocols using β-D-glucosyl Yariv reagent for comprehensive AGP characterization.

Mots clés: Arabinogalactan proteins; Cell wall; β-D-Glucosyl Yariv; Electrophoresis; Immunocytochemistry; Monoclonal antibodies; Root

Domaines: Biologie végétale; Biochimie [q-bio.BM]; Amélioration des plantes; Phytopathologie et phytopharmacie; Biologie moléculaire; Génomique, Transcriptomique et Protéomique [q-bio.GN]; Biologie cellulaire; Organisation et fonctions cellulaires [q-bio.SC]; Interactions cellulaires [q-bio.CB]; Biologie du développement; Biotechnologies

Romain Castilleux, Marc Ropitaux, Youssef Manasfi, Sophie Bernard, Maïté Vicré-Gibouin, et al.. Contributions to Arabinogalactan Protein Analysis. The Plant Cell Wall, 2149, Springer New York, pp.383-402, 2020, Methods in Molecular Biology, ⟨10.1007/978-1-0716-0621-6_22⟩. ⟨hal-03388397⟩