HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Comparative Structural and Functional Analyses of the Fusiform, Oval, and Triradiate Morphotypes of Phaeodactylum tricornutum Pt3 Strain

Ludovic Galas 1 Carole Burel 2 Damien Schapman 1 Marc Ropitaux 2 Sophie Bernard 2, 1 Magalie Bénard 1 Muriel Bardor 2
1 PRIMACEN - Plate-Forme de Recherche en Imagerie Cellulaire de Haute-Normandie
UNIROUEN - Université de Rouen Normandie, IRIB - Institute for Research and Innovation in Biomedicine, HeRacLeS - High-tech Research Infrastructures for Life Sciences
Abstract : The diatom Phaeodactylum tricornutum is a marine unicellular microalga that exists under three main morphotypes: oval, fusiform, and triradiate. Previous works have demonstrated that the oval morphotype of P. tricornutum Pt3 strain presents specific metabolic features. Here, we compared the cellular organization of the main morphotypes of the diatom P. tricornutum Pt3 strain through transmission electron and advanced light microscopies. The three morphotypes share similarities including spectral characteristics of the plastid, the location of the nucleus, the organization of mitochondria around the plastid as well as the existence of both a F-actin cortex, and an intracellular network of F-actin. In contrast, compared to fusiform and triradiate cells, oval cells spontaneously release proteins more rapidly. In addition, comparison of whole transcriptomes of oval versus fusiform or triradiate cells revealed numerous differential expression of positive and negative regulators belonging to the complex dynamic secretory machinery. This study highlights the specificities occurring within the oval morphotype underlying that the oval cells secrete proteins more rapidly.
Complete list of metadata

https://hal-normandie-univ.archives-ouvertes.fr/hal-03195703
Contributor : Arnaud Lehner Connect in order to contact the contributor
Submitted on : Monday, April 12, 2021 - 9:20:29 AM
Last modification on : Tuesday, May 10, 2022 - 2:48:02 PM
Long-term archiving on: : Tuesday, July 13, 2021 - 6:18:39 PM

File

2021-Galas-et-al-Front-Plant-S...
Publisher files allowed on an open archive

Identifiers

Citation

Ludovic Galas, Carole Burel, Damien Schapman, Marc Ropitaux, Sophie Bernard, et al.. Comparative Structural and Functional Analyses of the Fusiform, Oval, and Triradiate Morphotypes of Phaeodactylum tricornutum Pt3 Strain. Frontiers in Plant Science, Frontiers, 2021, 12, pp.584. ⟨10.3389/fpls.2021.638181⟩. ⟨hal-03195703⟩

Share

Metrics

Record views

56

Files downloads

48