Identification of N-glycan oligomannoside isomers in the diatom Phaeodactylum tricornutum

Rodolphe Dumontier (1) , Corinne Loutelier-Bourhis (2) , Marie-Laure Walet-Balieu (1) , Carole Burel (1) , Alain Mareck (1) , Carlos Afonso (2) , Patrice Lerouge (1) , Muriel Bardor (1)

1 Glyco-MEV - Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale
2 COBRA - Chimie Organique et Bioorganique : Réactivité et Analyse

Microalgae are emerging production systems for recombinant proteins like monoclonal antibodies. In this context, the characterization of the host cell N-glycosylation machinery and of the microalgae-made biopharmaceuticals, which are mainly glycoprotein-based products, requires efficient analytical methodologies dedicated to the profiling of the N-glycans. Herein, in order to gain knowledge regarding its N-glycosylation pathway, we profile the protein N-linked oligosaccharides isolated from the diatom Phaeodactylum tricornutum that has been used successfully to produce functional monoclonal antibodies. The combination of ion mobility spectrometry–mass Spectrometry and electrospray ionization-multistage tandem mass spectrometry allows us to decipher the detailed structure of the oligomannoside isomers and to demonstrate that the processing of the oligomannosides N-linked to proteins occurs in this diatom as reported in mammals. Therefore, P. tricornutum synthesizes human-like oligomannosides in contrast to other microalgae species. This represent an advantage as an alternative ecofriendly expression system to produce biopharmaceuticals used for human therapy

Mots clés: Glycans; Microalgae; Diatom; Phaeodactylum tricornutum; Oligomannosides; Isomers

Domaines: Polymères; Botanique; Biochimie [q-bio.BM]

Rodolphe Dumontier, Corinne Loutelier-Bourhis, Marie-Laure Walet-Balieu, Carole Burel, Alain Mareck, et al.. Identification of N-glycan oligomannoside isomers in the diatom Phaeodactylum tricornutum. Carbohydrate Polymers, 2021, 259, pp.117660. ⟨10.1016/j.carbpol.2021.117660⟩. ⟨hal-03141148⟩