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Identification of N-glycan oligomannoside isomers in the diatom Phaeodactylum tricornutum

Abstract : Microalgae are emerging production systems for recombinant proteins like monoclonal antibodies. In this context, the characterization of the host cell N-glycosylation machinery and of the microalgae-made biopharmaceuticals, which are mainly glycoprotein-based products, requires efficient analytical methodologies dedicated to the profiling of the N-glycans. Herein, in order to gain knowledge regarding its N-glycosylation pathway, we profile the protein N-linked oligosaccharides isolated from the diatom Phaeodactylum tricornutum that has been used successfully to produce functional monoclonal antibodies. The combination of ion mobility spectrometry–mass Spectrometry and electrospray ionization-multistage tandem mass spectrometry allows us to decipher the detailed structure of the oligomannoside isomers and to demonstrate that the processing of the oligomannosides N-linked to proteins occurs in this diatom as reported in mammals. Therefore, P. tricornutum synthesizes human-like oligomannosides in contrast to other microalgae species. This represent an advantage as an alternative ecofriendly expression system to produce biopharmaceuticals used for human therapy
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Submitted on : Monday, February 15, 2021 - 9:34:05 AM
Last modification on : Saturday, June 25, 2022 - 9:56:35 AM
Long-term archiving on: : Sunday, May 16, 2021 - 6:17:10 PM


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Rodolphe Dumontier, Corinne Loutelier-Bourhis, Marie-Laure Walet-Balieu, Carole Burel, Alain Mareck, et al.. Identification of N-glycan oligomannoside isomers in the diatom Phaeodactylum tricornutum. Carbohydrate Polymers, Elsevier, 2021, 259, pp.117660. ⟨10.1016/j.carbpol.2021.117660⟩. ⟨hal-03141148⟩



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