New Insights into Plant Extracellular DNA. A Study in Soybean Root Extracellular Trap
Abstract
exDNA is found in various organisms, including plants. However, plant exDNA has thus far received little attention related to its origin and role in the RET (root extracellular trap). In this study, we performed the first high-throughput genomic sequencing of plant exDNA from a Fabaceae with worldwide interest: soybean (Glycine max (L.) Merr.). The origin of this exDNA was first investigated in control condition, and the results show high-coverage on organelles (mitochondria/plastid) DNA relative to nuclear DNA, as well as a mix of coding and non-coding sequences. In the second part of this study, we investigated if exDNA release was modified during an elicitation with PEP-13 (a peptide elicitor from oomycete genus Phytophthora). Our results show that treatment of roots with PEP-13 does not affect the composition of exDNA.
Domains
Life Sciences [q-bio] Vegetal Biology Life Sciences [q-bio] Cellular Biology Life Sciences [q-bio] Cellular Biology Cell Behavior [q-bio.CB] Life Sciences [q-bio] Development Biology Life Sciences [q-bio] Biochemistry, Molecular Biology Biochemistry [q-bio.BM] Life Sciences [q-bio] Biochemistry, Molecular Biology Molecular biology Life Sciences [q-bio] Biotechnology Life Sciences [q-bio] Vegetal Biology Plant breeding Life Sciences [q-bio] Vegetal Biology Phytopathology and phytopharmacy Life Sciences [q-bio] Biochemistry, Molecular Biology Genomics [q-bio.GN] Life Sciences [q-bio] Cellular Biology Subcellular Processes [q-bio.SC]
Origin : Publisher files allowed on an open archive