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Journal articles
Root border cells and mucilage secretions of soybean, Glycine max (Merr) L.: characterization and role in interactions with the oomycete Phytophthora parasitica
Abstract : Root border cells (BCs) and their associated secretions form a protective structure termed the root extracellular trap (RET) that plays a major role in root interactions with soil borne microorganisms. In this study, we investigated the release and morphology of BCs of Glycine max using light and cryo-scanning electron microscopy (SEM). We also examined the occurrence of cell-wall glycomolecules in BCs and secreted mucilage using immunofluorescence microscopy in conjunction with anti-glycan antibodies. Our data show that root tips released three populations of BCs defined as spherical, intermediate and elongated cells. The mechanism of shedding seemed to be cell morphotype-specific. The data also show that mucilage contained pectin, cellulose, extracellular DNA, histones and two hemicellulosic polysaccharides, xyloglucan and heteromannan. The latter has never been reported previously in any plant root secretions. Both hemicellulosic polysaccharides formed a dense fibrillary network embedding BCs and holding them together within the mucilage. Finally, we investigated the effect of the RET on the interactions of root with the pathogenic oomycete Phytophthora parasitica early during infection. Our findings reveal that the RET prevented zoospores from colonizing root tips by blocking their entry into root tissues and inducing their lysis.
Cited literature [82 references]
https://hal-normandie-univ.archives-ouvertes.fr/hal-02975399
Contributor : Arnaud Lehner Connect in order to contact the contributor
Submitted on : Thursday, October 22, 2020 - 4:18:52 PM
Last modification on : Tuesday, May 10, 2022 - 2:48:02 PM
Long-term archiving on: : Saturday, January 23, 2021 - 7:49:38 PM
Contributor : Arnaud Lehner Connect in order to contact the contributor
Submitted on : Thursday, October 22, 2020 - 4:18:52 PM
Last modification on : Tuesday, May 10, 2022 - 2:48:02 PM
Long-term archiving on: : Saturday, January 23, 2021 - 7:49:38 PM
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2020-Ropitaux-et-al-Cells.pdf
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