Skip to Main content Skip to Navigation
Journal articles

Modeling undernutrition with enteropathy in mice

Abstract : Undernutrition is a global health issue leading to 1 out 5 all deaths in children under 5 years. Undernutrition is often associated with environmental enteric dysfunction (EED), a syndrome associated with increased intestinal permeability and gut inflammation. We aimed to develop a novel murine model of undernutrition with these EED features. Post-weaning mice were fed with low-protein diet (LP) alone or combined with a gastrointestinal insult trigger (indomethacin or liposaccharides). Growth, intestinal permeability and inflammation were assessed. LP diet induced stunting and wasting in post-weaning mice but did not impact gut barrier. We therefore combined LP diet with a single administration of indomethacin or liposaccharides (LPS). Indomethacin increased fecal calprotectin production while LPS did not. To amplify indomethacin effects, we investigated its repeated administration in addition to LP diet and mice exhibited stunting and wasting with intestinal hyperpermeability and gut inflammation. The combination of 3-weeks LP diet with repeated oral indomethacin administration induced wasting, stunting and gut barrier dysfunction as observed in undernourished children with EED. As noninvasive methods for investigating gut function in undernourished children are scarce, the present pre-clinical model provides an affordable tool to attempt to elucidate pathophysiological processes involved in EED and to identify novel therapeutic strategies.
Document type :
Journal articles
Complete list of metadatas

Cited literature [50 references]  Display  Hide  Download

https://hal-normandie-univ.archives-ouvertes.fr/hal-02964515
Contributor : Sabine Douville <>
Submitted on : Monday, October 12, 2020 - 3:24:18 PM
Last modification on : Thursday, October 15, 2020 - 1:59:43 PM

File

s41598-020-72705-0.pdf
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Emmeline Salameh, Marine Jarbeau, Fanny Morel, Mamane Zeilani, Moutaz Aziz, et al.. Modeling undernutrition with enteropathy in mice. Scientific Reports, 2020, 10 (1), pp.15581. ⟨10.1038/s41598-020-72705-0⟩. ⟨hal-02964515⟩

Share

Metrics

Record views

21

Files downloads

24