Combination of an Unbiased Amplification Method and a Resequencing Microarray for Detecting and Genotyping Equine Arteritis Virus

Aymeric Hans (1) , Delphine Gaudaire (1) , Jean-Claude Manuguerra (2) , Albertine Léon (3, 4) , Antoine Gessain (5) , Claire Laugier (1) , Nicolas Berthet (5) , Stéphan Zientara (6)

1 Laboratoire de pathologie équine de Dozulé
2 CIBU - Cellule d'Intervention Biologique d'Urgence - Laboratory for Urgent Response to Biological Threats
3 Laboratoire Frank Duncombe
4 LABÉO - LABÉO, Pôle d’analyses et de recherche de Normandie
5 EPVO (UMR_3569 / U-Pasteur_3) - Epidémiologie et Physiopathologie des Virus Oncogènes / Oncogenic Virus Epidemiology and Pathophysiology
6 VIRO - Virologie UMR1161

This study shows that an unbiased amplification method applied to equine arteritis virus RNA significantly improves the sensitivity of the real-time reverse transcription-quantitative PCR (RT-qPCR) recommended by the World Organization for Animal Health. Twelve viral RNAs amplified using this method were hybridized on a high-density resequencing microarray for effective viral characterization.

Domaines: Médecine vétérinaire et santé animale; Virologie

Aymeric Hans, Delphine Gaudaire, Jean-Claude Manuguerra, Albertine Léon, Antoine Gessain, et al.. Combination of an Unbiased Amplification Method and a Resequencing Microarray for Detecting and Genotyping Equine Arteritis Virus. Journal of Clinical Microbiology, 2014, 53 (1), pp.287-291. ⟨10.1128/JCM.01935-14⟩. ⟨hal-02946322⟩