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Combination of an Unbiased Amplification Method and a Resequencing Microarray for Detecting and Genotyping Equine Arteritis Virus

Aymeric Hans 1, * Delphine Gaudaire 1 Jean-Claude Manuguerra 2 Albertine Léon 3 Antoine Gessain 4 Claire Laugier 1 Nicolas Berthet 4 Stephan Zientara 5 
* Corresponding author
1 Laboratoire de Santé Animale [Goustranville]
2 CIBU - Cellule d'Intervention Biologique d'Urgence - Laboratory for Urgent Response to Biological Threats
3 Laboratoire Frank Duncombe
4 EPVO (UMR_3569 / U-Pasteur_3) - Epidémiologie et Physiopathologie des Virus Oncogènes / Oncogenic Virus Epidemiology and Pathophysiology
5 VIRO - Virologie UMR1161
Abstract : This study shows that an unbiased amplification method applied to equine arteritis virus RNA significantly improves the sensitivity of the real-time reverse transcription-quantitative PCR (RT-qPCR) recommended by the World Organization for Animal Health. Twelve viral RNAs amplified using this method were hybridized on a high-density resequencing microarray for effective viral characterization.
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https://hal-normandie-univ.archives-ouvertes.fr/hal-02946322
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Submitted on : Wednesday, September 23, 2020 - 9:17:59 AM
Last modification on : Friday, August 5, 2022 - 12:02:05 PM

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Aymeric Hans, Delphine Gaudaire, Jean-Claude Manuguerra, Albertine Léon, Antoine Gessain, et al.. Combination of an Unbiased Amplification Method and a Resequencing Microarray for Detecting and Genotyping Equine Arteritis Virus. Journal of Clinical Microbiology, American Society for Microbiology, 2014, 53 (1), pp.287-291. ⟨10.1128/JCM.01935-14⟩. ⟨hal-02946322⟩

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