Combination of an Unbiased Amplification Method and a Resequencing Microarray for Detecting and Genotyping Equine Arteritis Virus

Aymeric Hans (1) , Delphine Gaudaire (1) , Jean-Claude Manuguerra (2) , Albertine Léon (3) , Antoine Gessain (4) , Claire Laugier (1) , Nicolas Berthet (4) , Stéphan Zientara (5)

1 Laboratoire de pathologie équine de Dozulé
2 CIBU - Cellule d'Intervention Biologique d'Urgence - Laboratory for Urgent Response to Biological Threats
3 Laboratoire Frank Duncombe
4 EPVO (UMR_3569 / U-Pasteur_3) - Epidémiologie et Physiopathologie des Virus Oncogènes / Oncogenic Virus Epidemiology and Pathophysiology
5 VIRO - Virologie UMR1161

This study shows that an unbiased amplification method applied to equine arteritis virus RNA significantly improves the sensitivity of the real-time reverse transcription-quantitative PCR (RT-qPCR) recommended by the World Organization for Animal Health. Twelve viral RNAs amplified using this method were hybridized on a high-density resequencing microarray for effective viral characterization.

Domains: Life Sciences [q-bio]; Animal biology; Veterinary medicine and animal Health; Life Sciences [q-bio]; Microbiology and Parasitology; Virology

Aymeric Hans, Delphine Gaudaire, Jean-Claude Manuguerra, Albertine Léon, Antoine Gessain, et al.. Combination of an Unbiased Amplification Method and a Resequencing Microarray for Detecting and Genotyping Equine Arteritis Virus. Journal of Clinical Microbiology, 2014, 53 (1), pp.287-291. ⟨10.1128/JCM.01935-14⟩. ⟨hal-02946322⟩