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Poster communications

Synthesis, characterization and quantification by UHPLC-MS/MS of DNA adducts from 5 oxy-PAHs for the evaluation of their genotoxicity

Abstract : The exposome can be defined as the association of all the exposures to which one is subjected during his entire life. It was developed with the aim to understand the etiology of chronic diseases such as cancer. This concept highlights the need to evaluate environmental exposure in a more comprehensive manner. It can be realized thanks to the use of relevant biomarkers of exposure. Polycyclic Aromatic Hydrocarbons (PAHs) belong to a category of environmental pollutants to which humans are frequently exposed. It has been widely studied as important environmental pollutants because of their persistence, potential of bioaccumulation, and toxic effects. Different studies about toxicity of environmental pollutants demonstrate that toxicity of PAH fraction is generally upper than toxicity of the 16 priority PAH list. Consequently, other families such as nitrated PAHs (nitro-PAHs) and oxygenated PAHs (oxy-PAHs) complement this global toxicity. Oxy-PAHs are ubiquitous compounds in the environment and very few data are available on their toxicity. , In this study, 5 oxy-PAHs (e.g. 7,12-benz[a]anthracenequinone, 6H-benzo[cd]pyren-6-one, 7H-benz[de]anthracen-7-one, 1,2-naphthoquinone and 9-fluorenone) were selected on criteria of occurrence and potential toxicity. Research are focused more precisely on genotoxicity which encompasses alteration of DNA in connection with cancer risk. These compounds will be compared to benzo[a]pyrene (B[a]P), used as reference in terms of genotoxicity. We show here the evidence of cytotoxic and genotoxic effects of these compounds on human lung cells (BEAS-2B). Indeed, DNA adducts have been detected by 32P post-labelling for most of them. Adducts have been synthesized and purified. These DNA adducts were characterized and a UHPLC-MS/MS was developed to determine specific signature of oxy-PAHs exposure from white blood cells.
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https://hal-normandie-univ.archives-ouvertes.fr/hal-02477589
Contributor : Adeline Clerge <>
Submitted on : Thursday, February 13, 2020 - 2:47:56 PM
Last modification on : Wednesday, June 10, 2020 - 12:44:08 PM

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Adeline Clergé, Jérémie Le Goff, Isabelle Vaudorne, Stéphanie Lagadu, Raphaël Delépée. Synthesis, characterization and quantification by UHPLC-MS/MS of DNA adducts from 5 oxy-PAHs for the evaluation of their genotoxicity. 32nd International Symposium on Chromatography ISC 2018, Sep 2018, Cannes - Mandelieu, France. ⟨hal-02477589⟩

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