Skip to Main content Skip to Navigation
New interface
Journal articles

Evaluation of Lung Cell Toxicity of Surfactants for Inhalation Route

Abstract : Few data are available for excipients administered by inhalation route. This study evaluated the in vitro potential toxicity of three surfactants (Polysorbate 20, Polysorbate 80 and Poloxamer 188) by using an original air-liquid interface (ALI) method of exposure compared to liquid/liquid (L/L) model. Two cell toxicity tests were conducted on BEAS-2B cells, a human immortalized bronchial epithelial cell lines; measurement of Lactate Dehydrogenase activity and XTT cell proliferation assay. We found that Polysorbate 20 appeared to be more toxic than Polysorbate 80, Poloxamer 188. An increased toxicity of Polysorbate 20 in L/L system as shown in comparison to ALI exposure. A toxicity was also observed for polysorbate 80 but at higher concentrations and without difference between L/L and ALI exposure. No toxicity was observed for Poloxamer 188 at high concentrations. Poloxamer 188 seems to be the better candidate, out of the three tested, for galenic formulations designed to the inhalation route such as biotherapies. To evaluate the cytotoxicity of excipients for inhalation route the ALI exposure have to be used instead of L/L.
Document type :
Journal articles
Complete list of metadata

Cited literature [7 references]  Display  Hide  Download
Contributor : François SICHEL Connect in order to contact the contributor
Submitted on : Monday, June 29, 2020 - 12:12:52 PM
Last modification on : Tuesday, May 17, 2022 - 10:58:37 AM


Publication funded by an institution


Distributed under a Creative Commons Attribution 4.0 International License



F Lindenberg, François Sichel, Mathilde Lechevrel, R Respaud, Guillaume Saint-Lorant. Evaluation of Lung Cell Toxicity of Surfactants for Inhalation Route. Journal of Toxicology and Risk Assessment, 2019, 5 (1), pp.133. ⟨10.23937/2572-4061.1510022⟩. ⟨hal-02448735⟩



Record views


Files downloads