Chromosomal localization of three somatostatin genes in zebrafish. Evidence that the [Pro2]-somatostatin-14 isoform and cortistatin are encoded by orthologous genes

Hervé Tostivint (1, 2) , Lucille Joly (3) , Isabelle Lihrmann (1, 2) , Marc Ekker (3) , Hubert Vaudry (1, 2)

1 Neuroendocrinologie cellulaire et moléculaire
2 IFRMP 23 - Institut Fédératif de Recherches Multidisciplinaires sur les Peptides
3 University of Ottawa [Ottawa]

There is now evidence for the existence of two somatostatin genes in most vertebrate species, and even three somatostatin genes in teleosts. To help clarify the evolutionary relationships between the different somatostatin isoforms currently known, we characterized the somatostatin loci in a teleost species, the zebrafish Danio rerio, and compared them with the corresponding regions in the human and pufferfish genomes. The occurrence of three somatostatin genes, termed SS1, SS2 and SSII, has been previously demonstrated in the zebrafish. Radiation hybrid mapping assigned these three genes to linkage groups 15, 23 and 2, respectively. Conserved synteny of the zebrafish SS2 gene and the human cortistatin gene was revealed by comparative genomic analysis, indicating that mammalian cortistatin is orthologous to the SS2 variant of non-mammalian species. In contrast, using a similar approach, it was not possible to identify the evolutionary relationships between the atypical SSII gene of zebrafish and the other teleost SSII genes.

Domaines: Sciences du Vivant [q-bio]; Biochimie, Biologie Moléculaire; Organisation et fonctions cellulaires [q-bio.SC]

Hervé Tostivint, Lucille Joly, Isabelle Lihrmann, Marc Ekker, Hubert Vaudry. Chromosomal localization of three somatostatin genes in zebrafish. Evidence that the [Pro2]-somatostatin-14 isoform and cortistatin are encoded by orthologous genes. Journal of Molecular Endocrinology, 2004, 33 (3), pp.R1-R8. ⟨10.1677/jme.1.01602⟩. ⟨hal-02427151⟩