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Measurement of the neutralisation of equine influenza virus by impedancemetry

Abstract : Equine influenza virus (EIV) is an important respiratory pathogens of the horse. Beyond the welfare issue, equine influenza can significantly affect the equine industry. Vaccination is widely and effectively used to prevent EIV infection. Identification and validation of effective antiviral compounds would also increase our therapeutic arsenal against this pathogen. To date, the methods of choice to study neutralising antibody induced by vaccination (or natural infection) and/or activity of antiviral molecules are based on the infection of embryonated chicken eggs. This method is time consuming and requires the use of a significant number of eggs. The real-time measurement technology by xCELLigence® has been used to test several aspects of equine herpesviruses infection, replication, neutralization and inhibition in vitro. This presentation presents some preliminary data related to the use of impedancemetry to measure the efficacy of three antiviral compounds: zanamivir, memantin and a molecule derived from the amantadin. The impedancemetry may also be used to measure neutralising antibody activity in serum and could represent a potential alternative to embryonated eggs infection.
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Conference papers
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https://hal-normandie-univ.archives-ouvertes.fr/hal-02392929
Contributor : Flora Carnet <>
Submitted on : Wednesday, December 4, 2019 - 10:55:52 AM
Last modification on : Wednesday, October 21, 2020 - 11:30:03 AM

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  • HAL Id : hal-02392929, version 1

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Flora Carnet, Gabrielle Sutton, Côme Thieulent, Stéphane Pronost, Romain Paillot. Measurement of the neutralisation of equine influenza virus by impedancemetry. European Meeting of Equine Infectious Diseases, Nov 2019, Caen, France. ⟨hal-02392929⟩

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