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Prophylactic Injection of Recombinant Alpha-Enolase Reduces Arthritis Severity in the Collagen-Induced Arthritis Mice Model

Clément Guillou 1 Céline Derambure 2 Manuel Fréret 3 Mathieu Verdet 4 Gilles Avenel 5 Marie-Laure Golinski 5 Jean-Christophe Sabourin 6 François Le Loarer 7 Sahil Adriouch 3 Olivier Boyer 3 Thierry Lequerré 8 Olivier Vittecoq 8
1 UNIROUEN - Université de Rouen Normandie
2 GPMCND - Génomique et Médecine Personnalisée du Cancer et des Maladies Neuropsychiatriques
3 Physiopathologie et biothérapies des maladies inflammatoires et autoimmunes
4 UPMF IUG - Université Pierre Mendès France - Grenoble 2 - Institut d'urbanisme de Grenoble
5 UNIROUEN UFR Santé - UNIROUEN - UFR Santé
6 CHU Rouen
7 Département de Pathologie [CHU Rouen]
8 Service de rhumatologie [CHU Rouen]
Abstract : Objective To evaluate the ability of the glycolytic enzyme alpha-enolase (ENO1) or its immunodominant peptide (pEP1) to reduce the severity of CIA in DBA/1 mice when injected in a prophylactic way. Methods Mice were treated with mouse ENO1 or pEP1 one day prior to collagen II immunization. Clinical assessment was evaluated using 4 parameters (global and articular scores, ankle thickness and weight). Titers of serum anti-ENO1, anti-cyclic citrullinated peptides (antiCCP) and anti-CII (total IgG and IgG1/IgG2a isotypes) antibodies were measured by ELISA at different time-points. Disease activity was assessed by histological analysis of both anterior and hind paws at the end of experimentation. Results Prophylactic injection of 100 μg of ENO1 reduced severity of CIA. Serum levels of anti-CII antibodies were reduced in ENO1-treated mice. Concordantly, ENO1-treated mice joints presented less severe histological signs of arthritis. ENO1 did not induce a shift toward a Th2 response since IgG1/IgG2a ratio of anti-CII antibodies remained unchanged and IL-4 serum levels were similar to those measured in the control group. Conclusions Pre-immunization with ENO1 or its immunodominant peptide pEP1 reduces CIA severity at the clinical, immunological and histological levels. Effects of pEP1 were less pronounced. This immunomodulatory effect is associated with a reduction in anti-CII antibodies production but is not due to a Th1/Th2 shift.
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https://hal-normandie-univ.archives-ouvertes.fr/hal-02376205
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Submitted on : Friday, November 22, 2019 - 2:07:51 PM
Last modification on : Tuesday, November 16, 2021 - 11:55:50 AM

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COMUE-NORMANDIE | UNIROUEN | GPMCND | U1234PANTHER | UGA

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Clément Guillou, Céline Derambure, Manuel Fréret, Mathieu Verdet, Gilles Avenel, et al.. Prophylactic Injection of Recombinant Alpha-Enolase Reduces Arthritis Severity in the Collagen-Induced Arthritis Mice Model. PLoS ONE, Public Library of Science, 2015, 10 (8), pp.e0136359. ⟨10.1371/journal.pone.0136359⟩. ⟨hal-02376205⟩

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