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Journal articles
CRISPR-Barcoding for Intratumor Genetic Heterogeneity Modeling and Functional Analysis of Oncogenic Driver Mutations
Alexis Guernet
1
Sathish kumar Mungamuri
2
Dorthe Cartier
3
Ravi Sachidanandam
4
Anitha Jayaprakash
2
Sahil Adriouch
5
Myriam Vezain
6
Francoise Charbonnier
6
Guy Rohkin
2
Sophie Coutant
6
Shen Yao
2
Hassan Ainani
1
David Alexandre
7
Isabelle Tournier
6
Olivier Boyer
5
Stuart a. Aaronson
Youssef Anouar
7
Luca Grumolato
7
Alexis Guernet 1
Author
Sathish kumar Mungamuri 2
Author
Dorthe Cartier 3
Author
Ravi Sachidanandam 4
Author
Anitha Jayaprakash 2
Author
Sahil Adriouch 5
Author IdHAL : sahil-adriouch ResearcherId : http://www.researcherid.com/rid/G-8999-2012 ORCID : https://orcid.org/0000-0002-0265-7773
Myriam Vezain 6
Author IdHAL : myriam-vezain
Francoise Charbonnier 6
Author
Guy Rohkin 2
Author
Sophie Coutant 6
Author IdHAL : sophie-coutant
Shen Yao 2
Author
Hassan Ainani 1
Author
David Alexandre 7
Author
Isabelle Tournier 6
Author IdHAL : isabelle-tournier ORCID : https://orcid.org/0000-0002-5544-048X
Olivier Boyer 5
Author
Stuart a. Aaronson
Author
Youssef Anouar 7
Author
Luca Grumolato 7
Author IdHAL : luca-grumolato ORCID : https://orcid.org/0000-0001-8231-3032
1
DC2N - Différenciation et communication neuronale et neuroendocrine (Place Emile Blondel Faculté des Sciences et Techniques 76821 Mont-Saint-Aignan Cedex - France)
- UNIROUEN - Université de Rouen Normandie : U1239 (1, rue Thomas Becket 76821 Mont-Saint-Aignan Cedex - France)
- NU - Normandie Université (Esplanade de la Paix - CS 14032 - 14032 Caen Cedex 5 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1239 (101, rue de Tolbiac, 75013 Paris - France)
2
Department of Oncological Sciences [New York, NY, USA] (United States)
- MSSM - Icahn School of Medicine at Mount Sinai [New York] (1 Gustave L. Levy Place New York, NY 10029-5674 - United States)
3
Neuroendocrinologie cellulaire et moléculaire (Universite de Rouen 76821 MONT-SAINT-AIGNAN CEDEX - France)
- UNIROUEN - Université de Rouen Normandie : U413 (1, rue Thomas Becket 76821 Mont-Saint-Aignan Cedex - France)
- NU - Normandie Université (Esplanade de la Paix - CS 14032 - 14032 Caen Cedex 5 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U413 (101, rue de Tolbiac, 75013 Paris - France)
4
Cold Spring Harbor Laboratory (United States)
5
Physiopathologie et biothérapies des maladies inflammatoires et autoimmunes (22 boulevard Gambetta
76183 Rouen - France)
- UNIROUEN - Université de Rouen Normandie : U905 (1, rue Thomas Becket 76821 Mont-Saint-Aignan Cedex - France)
- NU - Normandie Université (Esplanade de la Paix - CS 14032 - 14032 Caen Cedex 5 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U905 (101, rue de Tolbiac, 75013 Paris - France)
6
GMFC - Génétique du cancer et des maladies neuropsychiatriques (22 Boulevard Gambetta 76183 Rouen Cedex - France)
- UNIROUEN - Université de Rouen Normandie (1, rue Thomas Becket 76821 Mont-Saint-Aignan Cedex - France)
- NU - Normandie Université (Esplanade de la Paix - CS 14032 - 14032 Caen Cedex 5 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U1079 (101, rue de Tolbiac, 75013 Paris - France)
7
DC2N - Différenciation et communication neuronale et neuroendocrine (Place Emile Blondel Faculté des Sciences et Techniques 76821 Mont-Saint-Aignan Cedex - France)
- UNIROUEN - Université de Rouen Normandie : U982 (1, rue Thomas Becket 76821 Mont-Saint-Aignan Cedex - France)
- NU - Normandie Université (Esplanade de la Paix - CS 14032 - 14032 Caen Cedex 5 - France)
- INSERM - Institut National de la Santé et de la Recherche Médicale : U982 (101, rue de Tolbiac, 75013 Paris - France)
Abstract : Intratumor genetic heterogeneity underlies the abilityof tumors to evolve and adapt to different environmental conditions. Using CRISPR/Cas9 technologyand specific DNA barcodes, we devised a strategyto recapitulate and trace the emergence of subpopulations of cancer cells containing a mutation ofinterest. We used this approach to model differentmechanisms of lung cancer cell resistance to EGFRinhibitors and to assess effects of combined drugtherapies. By overcoming intrinsic limitations of current approaches, CRISPR-barcoding also enablesinvestigation of most types of genetic modifications,including repair of oncogenic driver mutations.Finally, we used highly complex barcodes insertedat a specific genome location as a means of simultaneously tracing the fates of many thousands ofgenetically labeled cancer cells. CRISPR-barcodingis a straightforward and highly flexible method thatshould greatly facilitate the functional investigation ofspecific mutations, in a context that closely mimicsthe complexity of cancer.
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https://hal-normandie-univ.archives-ouvertes.fr/hal-02376161
Contributor : Sahil Adriouch <>
Submitted on : Friday, November 22, 2019 - 1:45:59 PM
Last modification on : Tuesday, June 30, 2020 - 3:32:45 AM
Contributor : Sahil Adriouch <>
Submitted on : Friday, November 22, 2019 - 1:45:59 PM
Last modification on : Tuesday, June 30, 2020 - 3:32:45 AM
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- HAL Id : hal-02376161, version 1
- DOI : 10.1016/j.molcel.2016.06.017
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Citation
Alexis Guernet, Sathish kumar Mungamuri, Dorthe Cartier, Ravi Sachidanandam, Anitha Jayaprakash, et al.. CRISPR-Barcoding for Intratumor Genetic Heterogeneity Modeling and Functional Analysis of Oncogenic Driver Mutations. Molecular Cell, Elsevier, 2016, 63 (3), pp.526-538. ⟨10.1016/j.molcel.2016.06.017⟩. ⟨hal-02376161⟩
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