Monitoring Expression and Enzyme Activity of Ecto-ARTCs

Stephan Menzel , Sahil Adriouch (1) , Peter Bannas , Friedrich Haag (2) , Friedrich Koch-Nolte (2)

1 Physiopathologie et biothérapies des maladies inflammatoires et autoimmunes
2 Institute for Immunology

Mammalian ARTCs are expressed as glycosylphosphatidylinositol (GPI)-anchored ectoenzymes (ARTC1–ARTC4) or secretory proteins (ARTC5) by different cell types. The ARTC2 enzymes catalyze mono-ADP-ribosylation of arginine residues in the extracellular domain of membrane proteins or secretory proteins. In this chapter we provide protocols to monitor the expression and activity of ARTCs on the cell membrane of living cells and in soluble form in biological fluids.

Keywords: ARTC; Ecto-ADP-ribosyltransferases; ADP-ribosylation; SDS-PAGE autoradiography; Flow cytometry; Near-infrared in vivo imaging

Domains: Life Sciences [q-bio]; Immunology; Life Sciences [q-bio]; Biochemistry, Molecular Biology

Stephan Menzel, Sahil Adriouch, Peter Bannas, Friedrich Haag, Friedrich Koch-Nolte. Monitoring Expression and Enzyme Activity of Ecto-ARTCs. Paul Chang. ADP-ribosylation and NAD+ utilizing enzymes : methods and protocols, Humana Press, pp.167-186, 2018, 978-1-4939-8587-6. ⟨10.1007/978-1-4939-8588-3_11⟩. ⟨hal-02375747⟩