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Monitoring Expression and Enzyme Activity of Ecto-ARTCs

Stephan Menzel Sahil Adriouch 1 Peter Bannas Friedrich Haag 2 Friedrich Koch-Nolte 2
1 Physiopathologie et biothérapies des maladies inflammatoires et autoimmunes
2 Institute for Immunology
Abstract : Mammalian ARTCs are expressed as glycosylphosphatidylinositol (GPI)-anchored ectoenzymes (ARTC1–ARTC4) or secretory proteins (ARTC5) by different cell types. The ARTC2 enzymes catalyze mono-ADP-ribosylation of arginine residues in the extracellular domain of membrane proteins or secretory proteins. In this chapter we provide protocols to monitor the expression and activity of ARTCs on the cell membrane of living cells and in soluble form in biological fluids.
Keywords : ADP-ribosylation Ecto-ADP-ribosyltransferases ARTC SDS-PAGE autoradiography Flow cytometry Near-infrared in vivo imaging
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https://hal-normandie-univ.archives-ouvertes.fr/hal-02375747
Contributor : Sahil Adriouch <>
Submitted on : Friday, November 22, 2019 - 10:42:28 AM
Last modification on : Friday, May 22, 2020 - 4:02:29 PM

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COMUE-NORMANDIE | UNIROUEN | U1234PANTHER

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Stephan Menzel, Sahil Adriouch, Peter Bannas, Friedrich Haag, Friedrich Koch-Nolte. Monitoring Expression and Enzyme Activity of Ecto-ARTCs. Paul Chang. ADP-ribosylation and NAD+ utilizing enzymes : methods and protocols, Humana Press, pp.167-186, 2018, 978-1-4939-8587-6. ⟨10.1007/978-1-4939-8588-3_11⟩. ⟨hal-02375747⟩

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