Lipase and acidic phosphatase from the psychrotrophic bacterium Pseudomonas fluorescens : Two enzymes whose synthesis is regulated by the growth temperature
Résumé
In the psychrotrophic bacterium Pseudomonas fluorescens, the production of several enzymes that otherwise differ in
their cell location, growth phase production and inducibility appeared to be similarly regulated by the growth temperature. In order
to determine the level of this regulation, the expression of the apo and lip genes encoding two of these enzymes, the acidic
phosphatase and lipase, respectively, was studied at different temperatures. Both genes were optimally expressed at 17.5°C, i.e., at
the optimal temperature of production of the enzymes; however, the low level of activity at the highest temperature could be due to
an additional post-transcriptional control.