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Adaptation of Pseudomonas aeruginosa to a pulsed light-induced stress

Abstract : Aims: Pulsed light (PL) technology is an efficient surface decontamination process. Used in low transmitted energy conditions, PL induces a stress that can be perceived by bacteria. The effect of such a PL stress was investigated on the highly environmental adaptable germ Pseudomonas aeruginosa PAO1. Methods and Results: Pulses of transmitted energy (fluence) reaching 1.8 J cm(-2) can kill 10(9) bacteria. Application of a lower sublethal PL dose allowed the bacteria to resist and survive more efficiently to a subsequent dose of PL. This sublethal dose was not increasing the mutation frequency of Ps. aeruginosa, but altered the abundance of 15 proteins as revealed by a global proteome analysis, including stress-induced proteins, phage-related proteins, energy and carbon metabolisms, cell motility, and transcription and translation regulators. Conclusions: A response to a low-energy PL dose takes place in Ps. aeruginosa, reducing the energy conversion systems, while increasing transcription and translation processes to produce proteins involved in chaperone mechanisms and phage-related proteins, probably to protect the bacterium against a new PL-induced stress. Significance and Impact of the Study: Taken together, these results suggest that a low-energy PL dose is sufficient to provoke adaptation of Ps. aeruginosa, leading to enhancing its resistance to a subsequent lethal treatment.
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https://hal-normandie-univ.archives-ouvertes.fr/hal-02366329
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Submitted on : Friday, November 15, 2019 - 6:44:08 PM
Last modification on : Monday, March 28, 2022 - 4:58:03 PM

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S. Massier, A. Rincé, O. Maillot, M.G.J. Feuilloley, N. Orange, et al.. Adaptation of Pseudomonas aeruginosa to a pulsed light-induced stress. Journal of Applied Microbiology, Wiley, 2012, 112 (3), pp.502-511. ⟨10.1111/j.1365-2672.2011.05224.x⟩. ⟨hal-02366329⟩

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