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Biocontrol of Soft Rot: Confocal Microscopy Highlights Virulent Pectobacterial Communication and Its Jamming by Rhodococcal Quorum-Quenching

Abstract : Confocal laser-scanning microscopy was chosen to observe the colonization and damage caused by the soft rot Pectobacterium atrosepticum and the protection mediated by the biocontrol agent Rhodococcus erythropolis. We developed dual-color re- porter strains suited for monitoring quorum-sensing and quorum-quenching activities leading to maceration or bio- control, respectively. A constitutively expressed cyan or red fluorescent protein served as a cell tag for plant colonization, while an inducible expression reporter system based on the green fluorescent protein gene enabled the simultaneous re- cording of signaling molecule production, detection, or deg- radation. The dual-colored pathogen and biocontrol strains were used to coinoculate potato tubers. At cellular quorum, images revealed a strong pectobacterial quorum-sensing ac- tivity, especially at the plant cell walls, as well as a concomitant rhodococcal quorum-quenching response, at both the single- cell and microcolony levels. The generated biosensors appear to be promising and complementary tools useful for molecular and cellular studies of bacterial communication and interference.
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https://hal-normandie-univ.archives-ouvertes.fr/hal-02356443
Contributor : Xavier Latour <>
Submitted on : Friday, November 8, 2019 - 5:19:16 PM
Last modification on : Wednesday, December 4, 2019 - 10:46:11 AM

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  • HAL Id : hal-02356443, version 1

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A Chane, C Barbey, M Robert, A Merieau, Y Konto-Ghiorghi, et al.. Biocontrol of Soft Rot: Confocal Microscopy Highlights Virulent Pectobacterial Communication and Its Jamming by Rhodococcal Quorum-Quenching. Molecular Plant-Microbe Interactions, American Phytopathological Society, 2019. ⟨hal-02356443⟩

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