Biochemical Characterisation and Immunohistochemical Localisation of the Secretogranin II-Derived Peptide EM66 in the Hypothalamus of the Jerboa (Jaculus orientalis): Modulation by Food Deprivation

Abstract : The neuroendocrine protein secretogranin II is the precursor of several neuropeptides, including secreto-neurin and a novel 66-amino acid peptide, EM66, the sequence of which has been highly conserved across the vertebrae phylum. The presence of EM66 has been detected in the adult and fetal human adrenal gland, as well as the rat pituitary and adrenal glands. The present study aimed to explore a possible neuroendocrine role of EM66 by analysing its occurrence and distribution within the jerboa hypothalamus, and its potential implication in the control of feeding behaviour. High-performance liquid chromatography analysis of jerboa hypothalamic extracts combined with a radioimmunoassay of EM66 revealed a single peak of immunoreactive material exhibiting the same retention time as recombinant EM66. Immuno-cytochemical labelling showed that EM66-producing neurones are widely distributed in several hypotha-lamic regions, including the preoptic area, the suprachiasmatic, supraoptic, parvocellular paraventricular and arcuate nuclei, and the lateral hypothalamus. Food deprivation for 5 days induced a significant increase in the number of EM66-containing neurones within the arcuate nucleus (105% increase) and the parvo-cellular aspect of the paraventricular nucleus (115% increase), suggesting that EM66 could be involved in the control of feeding behaviour and/or the response to stress associated with fasting. Altogether, these data reveal the physiological plasticity of the EM66 system in the hypothalamus and implicate this novel peptide in the regulation of neuroendocrine functions.
Liste complète des métadonnées

Littérature citée [33 références]  Voir  Masquer  Télécharger

https://hal-normandie-univ.archives-ouvertes.fr/hal-02342558
Contributeur : Maïté Montero-Hadjadje <>
Soumis le : mercredi 6 novembre 2019 - 09:32:06
Dernière modification le : samedi 9 novembre 2019 - 05:00:03

Lien texte intégral

Identifiants

Collections

Citation

M. Boutahricht, J. Guillemot, Maite Montero-Hadjadje, S. Bellafqih, S Ouezzani, et al.. Biochemical Characterisation and Immunohistochemical Localisation of the Secretogranin II-Derived Peptide EM66 in the Hypothalamus of the Jerboa (Jaculus orientalis): Modulation by Food Deprivation. Journal of Neuroendocrinology, Wiley, 2005, 17 (6), pp.372-378. ⟨10.1111/j.1365-2826.2005.01314.x⟩. ⟨hal-02342558⟩

Partager

Métriques

Consultations de la notice

9