Skip to Main content Skip to Navigation
Journal articles

Pro-hormone Secretogranin II Regulates Dense Core Secretory Granule Biogenesis in Catecholaminergic Cells

Abstract : Processes underlying the formation of dense core secretory granules (DCGs) of neuroendocrine cells are poorly understood. Here, we present evidence that DCG biogenesis is dependent on the secretory protein secretogranin (Sg) II, a member of the gra-nin family of pro-hormone cargo of DCGs in neuroendocrine cells. Depletion of SgII expression in PC12 cells leads to a decrease in both the number and size of DCGs and impairs DCG trafficking of other regulated hormones. Expression of SgII fusion proteins in a secretory-deficient PC12 variant rescues a regulated secretory pathway. SgII-containing dense core vesi-cles share morphological and physical properties with bona fide DCGs, are competent for regulated exocytosis, and maintain an acidic luminal pH through the V-type H ؉-translocating ATPase. The granulogenic activity of SgII requires a pH gradient along this secretory pathway. We conclude that SgII is a critical factor for the regulation of DCG biogenesis in neuroen-docrine cells, mediating the formation of functional DCGs via its pH-dependent aggregation at the trans-Golgi network.
Complete list of metadatas

Cited literature [88 references]  Display  Hide  Download

https://hal-normandie-univ.archives-ouvertes.fr/hal-02334607
Contributor : Maïté Montero-Hadjadje <>
Submitted on : Sunday, October 27, 2019 - 5:16:25 AM
Last modification on : Tuesday, April 7, 2020 - 3:36:04 PM
Long-term archiving on: : Tuesday, January 28, 2020 - 1:30:36 PM

File

J. Biol. Chem.-2010-Courel-100...
Files produced by the author(s)

Identifiers

Collections

Citation

Maïté Courel, Alex Soler-Jover, Juan Rodriguez-Flores, Sushil Mahata, Salah Elias, et al.. Pro-hormone Secretogranin II Regulates Dense Core Secretory Granule Biogenesis in Catecholaminergic Cells. Journal of Biological Chemistry, American Society for Biochemistry and Molecular Biology, 2010, ⟨10.1074/jbc.M109.064196⟩. ⟨hal-02334607⟩

Share

Metrics

Record views

76

Files downloads

135