Skip to Main content Skip to Navigation
Journal articles

The long coiled-coil protein NECC2 is associated to caveolae and modulates NGF/TrkA signaling in PC12 cells [corrected].

Abstract : TrkA-mediated NGF signaling in PC12 cells has been shown to be compartimentalized in specialized microdomains of the plasma membrane, the caveolae, which are organized by scaffold proteins including the member of the caveolin family of proteins, caveolin-1. Here, we characterize the intracellular distribution as well as the biochemical and functional properties of the neuroendocrine long coiled-coil protein 2 (NECC2), a novel long coiled-coil protein selectively expressed in neuroendocrine tissues that contains a predicted caveolin-binding domain and displays structural characteristics of a scaffolding factor. NECC2 distributes in caveolae, wherein it colocalizes with the TrkA receptor, and behaves as a caveolae-associated protein in neuroendocrine PC12 cells. In addition, stimulation of PC12 cells with nerve growth factor (NGF) increased the expression and regulated the distribution of NECC2. Interestingly, knockdown as well as overexpression of NECC2 resulted in a reduction of NGF-induced phosphorylation of the TrkA downstream effector extracellular signal-regulated kinases 1 and 2 (ERK1/ERK2) but not of Akt. Altogether, our results identify NECC2 as a novel component of caveolae in PC12 cells and support the contribution of this protein in the maintenance of TrkA-mediated NGF signaling.
Complete list of metadatas

Cited literature [60 references]  Display  Hide  Download

https://hal-normandie-univ.archives-ouvertes.fr/hal-02334374
Contributor : Maïté Montero-Hadjadje <>
Submitted on : Friday, January 31, 2020 - 11:03:38 PM
Last modification on : Wednesday, August 26, 2020 - 10:26:02 AM
Long-term archiving on: : Friday, May 1, 2020 - 6:07:20 PM

File

islandora_63884.pdf
Publisher files allowed on an open archive

Identifiers

Citation

Alberto Díaz-Ruiz, Yoana Rabanal-Ruiz, Andrés Trávez, Francisco Gracia-Navarro, David Cruz-García, et al.. The long coiled-coil protein NECC2 is associated to caveolae and modulates NGF/TrkA signaling in PC12 cells [corrected].. PLoS ONE, Public Library of Science, 2013, 8 (9), pp.e73668. ⟨10.1371/journal.pone.0073668⟩. ⟨hal-02334374⟩

Share

Metrics

Record views

111

Files downloads

188