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Journal articles
Simultaneous detection of the protozoan parasites Toxoplasma, Cryptosporidium and Giardia in food matrices and their persistence on basil leaves
Jeanne Hohweyer
1
Catherine Cazeaux
2
Emmanuelle Travaillé
3
Emilie Languet
2
Aurélien Dumètre
4
Dominique Aubert
1
Christine Terryn
5
Jitender Dubey
6
Nadine Azas
4
Maryline Houssin
3
Favennec Loïc
1
Isabelle Villena
1
Stéphanie La Carbona
2
5
PICT - Plateforme en Imagerie Cellulaire et Tissulaire
URCA - Université de Reims Champagne-Ardenne, SFR CAP Santé (Champagne-Ardenne Picardie Santé)
Jeanne Hohweyer 1
Author
Catherine Cazeaux 2
Author
Emmanuelle Travaillé 3
Author
Emilie Languet 2
Author
Aurélien Dumètre 4
Author
Dominique Aubert 1
Author
Christine Terryn 5
Author
Jitender Dubey 6
Author
Stéphanie La Carbona 2
Author
1
PROTAL - Protozooses Transmises par l'Alimentation (Cryptosporidiose, Giardose et Toxoplasmose) : Mode de Contamination et Pathogénie (PROTAL) - EA 3800 (UFR MEDECINE
Laboratoire Parasitologie-Mycologie
51 rue Cognac Jay
51095 REIMS CEDEX - France)
- ANSES - Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail : EA 3800 (14 rue Pierre et Marie Curie 94701 MAISONS-ALFORT Cedex - France)
- UNIROUEN - Université de Rouen Normandie : EA 3800 (1, rue Thomas Becket 76821 Mont-Saint-Aignan Cedex - France)
- NU - Normandie Université (Esplanade de la Paix - CS 14032 - 14032 Caen Cedex 5 - France)
- SFR CAP Santé (Champagne-Ardenne Picardie Santé) (Faculté de Médecine de Reims, 51, rue Cognacq Jay, CS 30018,
51095 Reims Cedex - France)
- URCA - Université de Reims Champagne-Ardenne : FED 4231 (2 avenue Robert Schuman, 51100 Reims. - France)
- UPJV - Université de Picardie Jules Verne : FED 4231 (Chemin du Thil - 80000 Amiens - France)
- URCA - Université de Reims Champagne-Ardenne : EA 3800 (2 avenue Robert Schuman, 51100 Reims. - France)
2
Actalia [Saint-Lô] (310 Rue du Père Popiełujko, 50000 Saint-Lô - France)
3
LABÉO - LABÉO, Pôle d’analyses et de recherche de Normandie (1 Route de Rosel 14280 Saint-Contest - France)
4
IP-TPT - Infections Parasitaires : Transmission, Physiopathologie et Thérapeutiques (Faculté de Pharmacie
27 bd Jean Moulin
CS 30064
13385 Marseille - France)
- IRD - Institut de Recherche pour le Développement : UMR MD3 (Siège Le Sextant 44, bd de Dunkerque CS 90009 13572 Marseille cedex 02 - France)
- AMU - Aix Marseille Université : UMR MD3 (Aix-Marseille Université Jardins du Pharo 58 Boulevard Charles Livon 13284 Marseille cedex 7 - France)
- APHM - Assistance Publique - Hôpitaux de Marseille : UMR MD3 (Direction générale AP-HM 80, rue Brochier 13 354 Marseille Cedex 5 - France)
- Service de Santé des Armées : UMR MD3 (France)
5
PICT - Plateforme en Imagerie Cellulaire et Tissulaire (Reims - France)
- URCA - Université de Reims Champagne-Ardenne (2 avenue Robert Schuman, 51100 Reims. - France)
- SFR CAP Santé (Champagne-Ardenne Picardie Santé) (Faculté de Médecine de Reims, 51, rue Cognacq Jay, CS 30018,
51095 Reims Cedex - France)
- URCA - Université de Reims Champagne-Ardenne : FED 4231 (2 avenue Robert Schuman, 51100 Reims. - France)
- UPJV - Université de Picardie Jules Verne : FED 4231 (Chemin du Thil - 80000 Amiens - France)
6
Animal Parasitic Diseases Laboratory [Beltsville, USA] (10300 BALTIMORE AVENUE
BLDG. 007, RM. 301, BARC-WEST
Beltsville, MD 20705 - United States)
- USDA - United States Department of Agriculture (United States)
- USDA Agricultural Research Service [Beltsville, Maryland] (Beltsville Agricultural Research Center
10300 Baltimore Avenue
Bldg. 003, Rm. 231, BARC-West
Beltsville, MD 20705 - United States)
- USDA-ARS : Agricultural Research Service (United States)
Abstract : Toxoplasma gondii, Cryptosporidium spp. and Giardia intestinalis are emerging pathogen parasites in the food domain. However, without standardized methods for their detection in food matrices, parasitic foodborne outbreaks remain neglected. In this study, a new immunomagnetic separation assay (IMS Toxo) targeting the oocyst's wall of T. gondii was developed using a specific purified monoclonal antibody. Performance of this IMS Toxo coupled to microscopic and qPCR analyses was evaluated in terms of limit of detection (LOD) and recovery rate (RR) on: i) simple matrix (LOD ¼ 5 oocysts; RR between 5 and 56%); ii) raspberries and basil (LOD ¼ 33 oocysts/g; RR between 0.2 and 35%). Finally, to simultaneously extract the three protozoa from these food matrices, T. gondii oocysts were directly concentrated (without IMS Toxo) from the supernatant of the IMS of Cryptosporidium and Giardia (oo)cysts. This strategy associated to qPCR detection led to LOD <1 to 3 (oo)cysts/g and RR between 2 and 35%. This procedure was coupled to RT-qPCR analyses and showed that the three protozoa persisted on the leaves of basil and remained viable following storage at 4 C for 8 days. These data strengthen the need to consider these protozoa in food safety.
Document type :
Journal articles
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Complete list of metadata
https://hal-normandie-univ.archives-ouvertes.fr/hal-02317870
Contributor : Venceslas Villier Connect in order to contact the contributor
Submitted on : Thursday, October 24, 2019 - 9:39:57 AM
Last modification on : Wednesday, November 3, 2021 - 6:18:48 AM
Contributor : Venceslas Villier Connect in order to contact the contributor
Submitted on : Thursday, October 24, 2019 - 9:39:57 AM
Last modification on : Wednesday, November 3, 2021 - 6:18:48 AM
Identifiers
- HAL Id : hal-02317870, version 1
- DOI : 10.1016/j.fm.2016.01.002
Collections
COMUE-NORMANDIE | SSA | ANSES | ESCAPE-REIMS | URCA | UNIROUEN | UNIV-AMU | ESCAPE-ROUEN | U-PICARDIE
Citation
Jeanne Hohweyer, Catherine Cazeaux, Emmanuelle Travaillé, Emilie Languet, Aurélien Dumètre, et al.. Simultaneous detection of the protozoan parasites Toxoplasma, Cryptosporidium and Giardia in food matrices and their persistence on basil leaves. Food Microbiology, Elsevier, 2016, 57, pp.36-44. ⟨10.1016/j.fm.2016.01.002⟩. ⟨hal-02317870⟩
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