Development of preservative-free nanoparticles-based emulsions: Effects of NP surface properties and sterilization process

Abstract : Model emulsions were developed with or without commercial titanium dioxide nanoparticles (NP) carrying various surface treatments in order to get close physicochemical properties whatever the NP surface polarity (hydrophilic and hydrophobic). Rheology and texturometry highlighted that the macroscopic properties of the three formulated emulsions were similar. However, characterizations by optical microscopy, static light scattering and zetametry showed that their microstructures reflected the diversity of the incorporated NP surface properties. In order to use these model emulsions as tools for biological evaluations of the NP in use, they had to show the lowest initial microbiological charge and, specifically for the NP-free emulsion, the lowest bactericidal effect. Hence, formulae were developed preservative-free and a thermal sterilization step was conducted. Efficiency of the sterilization and its impact on the emulsion integrity were monitored. Results highlighted the effect of the NP surface properties: only the control emulsion and the emulsion containing hydrophilic NP fulfilled both requirements. To ensure the usability of these model emulsions as tools to evaluate the 'NP effect' on representative bacteria of the skin microflora (S. aureus and P. fluorescens), impact on the bacterial growth was measured on voluntary inoculated formulae.
Liste complète des métadonnées

Littérature citée [28 références]  Voir  Masquer  Télécharger

https://hal-normandie-univ.archives-ouvertes.fr/hal-02310892
Contributeur : Nicolas Hucher <>
Soumis le : jeudi 24 octobre 2019 - 12:09:04
Dernière modification le : mardi 29 octobre 2019 - 10:28:01

Fichier

Rowenczyk et al_2016_Internati...
Fichiers produits par l'(les) auteur(s)

Identifiants

Citation

Laura Rowenczyk, Celine Picard, Cécile Duclairoir-Poc, Nicolas Hucher, Nicole Orange, et al.. Development of preservative-free nanoparticles-based emulsions: Effects of NP surface properties and sterilization process. International Journal of Pharmaceutics, Elsevier, 2016, 510 (1), pp.125-134. ⟨10.1016/j.ijpharm.2016.06.014⟩. ⟨hal-02310892⟩

Partager

Métriques

Consultations de la notice

21

Téléchargements de fichiers

13