Determination of galactose and mannose residues in natural galactomannans using a fast and efficient high-performance liquid chromatography/UV detection

Abstract : The present work describes the validation of an easy, fast and efficient precolumn derivatization method for the quantification of oligosides, mannose and galactose obtained by degradation of galactomannans. This work combines an acid hydrolysis and an enzymatic degradation of natural galactomannans with the quantification of released residues by reversed-phase HPLC-UV, the most usual HPLC system in laboratories. In case of enzymatic degradation, mannotetraose has been detected and quantified for the first time, and an application to the evaluation of the galactosyl distribution in galactomannans is proposed. After an acidic hydrolysis, this method also allowed to obtain the mannose/galactose (M/G) ratio.
Type de document :
Article dans une revue
Liste complète des métadonnées

https://hal-normandie-univ.archives-ouvertes.fr/hal-02310799
Contributeur : Nicolas Hucher <>
Soumis le : jeudi 24 octobre 2019 - 11:42:13
Dernière modification le : jeudi 24 octobre 2019 - 11:42:15

Identifiants

Collections

Citation

N. Tapie, C. Malhiac, Nicolas Hucher, M. Grisel. Determination of galactose and mannose residues in natural galactomannans using a fast and efficient high-performance liquid chromatography/UV detection. Journal of Chromatography A, Elsevier, 2008, 1181 (1-2), pp.45-50. ⟨10.1016/j.chroma.2007.12.027⟩. ⟨hal-02310799⟩

Partager

Métriques

Consultations de la notice

13