Electrografting of diazonium salt for SPR application

Flavia Fioresi (1) , A. Rouleau (2) , K. Maximova (2) , Julien Vieillard (1) , W. Boireau (2) , C. Elie Caille (2) , Cécile Soulignac (1) , R. Zeggari (2) , T. Clamens (3) , Olivier Lesouhaitier (3) , Nadine Mofaddel (1) , Franck Le Derf (1)

1 COBRA - Chimie Organique et Bioorganique : Réactivité et Analyse
2 FEMTO-ST - Franche-Comté Électronique Mécanique, Thermique et Optique - Sciences et Technologies (UMR 6174)
3 LMSM - Laboratoire de Microbiologie Signaux et Microenvironnement

A general method to develop diazonium-based biochip for SPRi analysis is presented. The electrografting of carboxybenzene diazonium salt is optimized in order to fit with the requirement of the SPR technology. The influence of the surface preparation on plasmon quality is discussed and the performance of this original modified biochip is investigated for determining the binding constants between circulating ovalbumin and spotted anti-ovalbumin antibody. The kinetic constants are calculated and compared with literature and similar performances are reached confirming that diazonium based biochip is a good complement to commercial SPRi sensor.

Domains: Chemical Sciences; Material chemistry

Flavia Fioresi, A. Rouleau, K. Maximova, Julien Vieillard, W. Boireau, et al.. Electrografting of diazonium salt for SPR application. Materials Today: Proceedings, 2019, 6, pp.340-344. ⟨10.1016/j.matpr.2018.10.428⟩. ⟨hal-02308928⟩