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Sex-dependent impact of Roundup on the rat gut microbiome

Abstract : A growing body of research suggests that dysbiosis of the gut microbiota induced by environmental pollutants, such as pesticides, could have a role in the development of metabolic disorders. We have examined the long-term effects of 3 doses of the Roundup(R) herbicide (made of glyphosate and formulants) on the gut microbiota in male and female Sprague-Dawley rats. A total of 141 bacteria families were identified by a 16S sequencing analysis approach. An OPLS-DA analysis revealed an increased Bacteroidetes family S24-7 and a decreased Lactobacillaceae in 8 out of the 9 females treated with 3 different doses of R (n = 3, for each dose). These effects were confirmed by repetitive sequence-based PCR fingerprinting showing a clustering of treated females. A culture-based method showed that R had a direct effect on rat gut microbiota. Cultivable species showed different sensitivities to R, including the presence of a high tolerant or resistant strain identified as Escherichia coli by 16S rRNA sequencing. The high tolerance of this E. Coli strain was explained by the absence of the EPSPS gene (coding glyphosate target enzyme) as shown by DNA amplification. Overall, these gut microbiome disturbances showed a substantial overlap with those associated with liver dysfunction in other studies. In conclusion, we revealed that an environmental concentration of R (0.1 ppb) and other two concentrations (400 ppm and 5,000 ppm) have a sex-dependent impact on rat gut microbiome composition and thus warrants further investigation .
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Soumis le : mardi 17 mars 2020 - 16:23:05
Dernière modification le : mercredi 18 mars 2020 - 11:02:55


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Veronica Lozano, Nicolas Defarge, Louis-Marie Rocque, Robin Mesnage, Didier Hennequin, et al.. Sex-dependent impact of Roundup on the rat gut microbiome. Toxicology Reports, Elsevier, 2018, 5, pp.96-107. ⟨10.1016/j.toxrep.2017.12.005⟩. ⟨hal-02294902⟩



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