Long-Lasting Cerebral Vasospasm, Microthrombosis, Apoptosis and Paravascular Alterations Associated with Neurological Deficits in a Mouse Model of Subarachnoid Hemorrhage

Mohamad El Amki; Martine Dubois; Antoine Lefevre-Scelles; Nicolas Magné; Mélanie Roussel; Thomas Clavier; Pierre-Olivier Guichet; Emmanuel Gerardin; Vincent Compère; Hélène Castel

Long-Lasting Cerebral Vasospasm, Microthrombosis, Apoptosis and Paravascular Alterations Associated with Neurological Deficits in a Mouse Model of Subarachnoid Hemorrhage

Mohamad El Amki ¹ Martine Dubois ¹ Antoine Lefevre-Scelles ^{1, 2, 3} Nicolas Magné ^{4, 1} Mélanie Roussel ^{1, 5} Thomas Clavier ¹ Pierre-Olivier Guichet ¹ Emmanuel Gerardin ^{6, 1} Vincent Compère ^{1, 3} Hélène Castel ¹

1 DC2N - Différenciation et communication neuronale et neuroendocrine

2 IRIB - Institute for Research and Innovation in Biomedicine

3 Service de soins intensifs [CHU Rouen]

4 Institut de Cancérologie Lucien Neuwirth

5 CHU Rouen

6 Service d'imagerie médicale [Rouen]

Abstract : Subarachnoid hemorrhage (SAH) is a devastating disease with high mortality and morbidity. Long-term cognitive and sensorimotor deficits are serious complications following SAH but still not well explained and described in mouse preclinical models. The aim of our study is to characterize a well-mastered SAH murine model and to establish developing pathological mechanisms leading to cognitive and motor deficits, allowing identification of specific targets involved in these long-term troubles. We hereby demonstrate that the double blood injection model of SAH induced long-lasting large cerebral artery vasospasm (CVS), microthrombosis formation and cerebral brain damage including defect in potential paravascular diffusion. These neurobiological alterations appear to be associated with sensorimotor and cognitive dysfunctions mainly detected 10 days after the bleeding episode. In conclusion, this characterized model of SAH in mice, stressing prolonged neurobiological pathological mechanisms and associated sensitivomotor deficits, will constitute a validated preclinical model to better decipher the link between CVS, long-term cerebral apoptosis and cognitive disorders occurring during SAH and to allow investigating novel therapeutic approaches in transgenic mice.

Keywords : Vasospasm Hemorrhage Microthrombi Sensitivomotor deficits Mice

Type de document :

Article dans une revue

Domaine :

Sciences cognitives

Sciences cognitives / Neurosciences

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https://hal-normandie-univ.archives-ouvertes.fr/hal-02280048
Contributeur : Hélène Castel <>
Soumis le : jeudi 5 septembre 2019 - 19:45:37
Dernière modification le : vendredi 27 septembre 2019 - 12:05:46

Long-Lasting Cerebral Vasospasm, Microthrombosis, Apoptosis and Paravascular Alterations Associated with Neurological Deficits in a Mouse Model of Subarachnoid Hemorrhage

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Long-Lasting Cerebral Vasospasm, Microthrombosis, Apoptosis and Paravascular Alterations Associated with Neurological Deficits in a Mouse Model of Subarachnoid Hemorrhage

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