Equine PBMC cytokines profile and efficacy of a Parapoxvirus ovis based-immunomodulator after in vitro α- and γ-EHV infection

Abstract : Horses are usually infected during the first months of life by several equine herpesviruses, such as EHV-1, EHV-4 or EHV-2. The persistence of these respiratory viruses through establishment of latency, often characterised by an absence of clinical signs, represents a real risk to the horse population. Reactivation of latent EHV could cause disease in both carriers and naive animals in contact. A better understanding of the immune response to EHV infection is necessary to improve our methods of prevention and decrease the risk of transmission. The objectives of this study were 1/ to characterise the cytokine gene expression profile of PBMC after in vitro EHV infection (EHV-1, EHV-4 and EHV-2) and 2/ To determine the efficacy of the inactivated Parapoxvirus ovis (iPPVO), a known immunomodulator in horses , against these same viruses. PBMC were isolated from 3 horses and infected in vitro with EHV-1, EHV-4 or EHV-2, in the presence or absence of iPPVO. In vitro culture of PBMC with EHV-1, EHV-4, EHV-2 and iPPVO induced a significant increase of IFN-α, IFN-β and IFN-γ gene expression. Moreover, EHV-2 induced a significant increase of IL-1β, IL-6 and TNF-α mRNA. The presence of iPPVO induced an earlier and stronger expression of IFN-α, IFN-β and IFN-γ during EHV infection. The presence of iPPVO reduced also the inflammatory response induced by EHV-2. In conclusion, this study suggests that the presence of iPPVO potentiates the development of the immune response during infection EHV in vitro.
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Contributeur : Erika Hue <>
Soumis le : mercredi 28 août 2019 - 17:27:22
Dernière modification le : lundi 21 octobre 2019 - 14:30:08

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E. Hue, Eric Richard, C. Fortier, G. Fortier, R. Paillot, et al.. Equine PBMC cytokines profile and efficacy of a Parapoxvirus ovis based-immunomodulator after in vitro α- and γ-EHV infection. Journal of Equine Veterinary Science, WB Saunders, 2016, Part of special issue: 10th International Equine Infectious Diseases Conference, 39, pp.S69. ⟨10.1016/j.jevs.2016.02.149⟩. ⟨hal-02273324⟩

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