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Poster communications

In vitro alpha- and gamma-EHV infection : Equine PBMC cytokines profile and efficacy of a Parapoxvirus ovis based-immunomodulator

Abstract : Horses are usually infected during the first months of life by several equine herpesviruses, such as EHV-1, EHV-4 or EHV-2. The persistence of these respiratory viruses through establishment of latency, often characterised by an absence of clinical signs, represents a real risk to the horse population. A better understanding of the immune response after EHV infection is necessary to improve our methods of prevention and decrease the risk of transmission. The objectives of this study were 1/ to characterise the cytokine gene expression profile of PBMC after in vitro EHV infection (EHV-1, EHV-4 and EHV-2) and 2/ To determine the efficacy of the inactivated Parapoxvirus ovis (iPPVO), a known immunomodulator in horses, against these three viruses.PBMC isolated from 3 horses were infected in vitro with EHV-1, EHV-4 or EHV-2, in the presence or absence of iPPVO. Cytokine expression profiles (IFN-alpha, IFN-beta, IFN-gamma, TNF-alpha, IL-6, IL-10) were measured by qRT-PCR.In vitro culture of PBMC after EHV-1, EHV-4 or EHV-2 infection in the presence of iPPVO induced a significant increase of IFN-alpha, IFN-beta and IFN-gamma gene expression. Moreover, EHV-2 induced a significant increase of IL-1beta, IL-6 and TNF-alpha mRNA. The presence of iPPVO induced an earlier and stronger expression of IFN-alpha, IFN-beta and IFN-gamma during EHV infections. The presence of iPPVO also reduced the inflammatory response induced by EHV-2. In conclusion, this study suggests that the presence of iPPVO potentiates the development of the immune response during EHV infection in vitro.
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Poster communications
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https://hal-normandie-univ.archives-ouvertes.fr/hal-02271567
Contributor : Erika Hue <>
Submitted on : Tuesday, August 27, 2019 - 9:26:57 AM
Last modification on : Wednesday, October 14, 2020 - 4:10:45 AM

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  • HAL Id : hal-02271567, version 1

Citation

Erika Hue, Eric Richard, Christine Fortier, Guillaume Fortier, Romain Paillot, et al.. In vitro alpha- and gamma-EHV infection : Equine PBMC cytokines profile and efficacy of a Parapoxvirus ovis based-immunomodulator. 5th Veterinary Herpesvirus Symposium of the European Society For Veterinary Virology (ESVV), Jul 2017, Ghent, Belgium. ⟨hal-02271567⟩

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