Cloning, sequencing and characterization of the ccpA gene from Enterococcus faecalis
Résumé
Enzymes involved in the metabolism of complex carbon and energy sources are unnecessary under conditions of
abundant, readily metabolisable nutrients such as glucose or fructose. The repression of these enzymes by glucose has been
termed carbon catabolite repression. Mechanisms involved in the carbon catabolite repression in Gram-positive bacteria are
known to differ from those of Gram-negative bacteria such as Escherichia coli. It appears to be mediated by transcriptional
repression, requiring trans-acting CcpA, a member of the LacI–GalR family of bacterial regulatory proteins and a cis-acting
consensus sequence, designated cre. Here, we report the cloning and characterisation of the chromosomal ccpA gene from
Enterococcus faecalis JH2-2. This gene is predicted to encode a 333 amino acids protein with nearly 75% identity to CcpA
of Lactobacillus casei. 2000 Elsevier Science B.V. All rights reserved.