Biomonitoring of urine mutagenicity with the Ames test: improvement of the extraction/concentration method

Veronique Andre (1) , Pierre Lebailly (1) , Edwige Deslandes (1) , Michel Henry-Amar (2) , Pascal Gauduchon (1)

1 GRECAN - Groupe Régional d'Etudes sur le CANcer
2 Calvados Cancer Registry

Comparative extraction efficiency of the pre-packed Bakerbond-spe-SDB-1 resin and of Amberlite-AD2 (XAD-2) resin, for the preparation of urine extracts in biomonitoring studies. Urine extracts were prepared in parallel with the Bakerbond column and with the classical XAD-2 resin from urines (1) spiked with mutagenic chemicals, (2) collected from patients after chemotherapy, and (3) from smokers. Mutagenic activities were evaluated on Salmonella typhimurium tester strains TA97a, TA98, TA100 and TA102 with and without S9 mix. Mutagenic activities obtained with Bakerbond extracts were almost always higher or at least equivalent to those prepared on XAD-2 resin. Similar results were observed for the three urine sample groups. When fully validated, the use of the pre-packed columns will be more convenient and time-saving for large population studies.

Domaines: Sciences du Vivant [q-bio]

Veronique Andre, Pierre Lebailly, Edwige Deslandes, Michel Henry-Amar, Pascal Gauduchon. Biomonitoring of urine mutagenicity with the Ames test: improvement of the extraction/concentration method. Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 2002, 520 (1-2), pp.199-205. ⟨10.1016/s1383-5718(02)00172-9⟩. ⟨hal-02186898⟩