Riboflavin photoactivation by upconversion nanoparticles for cancer treatment

E.V. Khaydukov; K.E. Mironova; V.A. Semchishen; A.N. Generalova; A.V. Nechaev; D.A. Khochenkov; E.V. Stepanova; O.I. Lebedev; A.V. Zvyagin; S.M. Deyev; V.Ya. Panchenko

Riboflavin photoactivation by upconversion nanoparticles for cancer treatment

E.V. Khaydukov ¹ K.E. Mironova ¹ V.A. Semchishen ¹ A.N. Generalova ¹ A.V. Nechaev ¹ D.A. Khochenkov ¹ E.V. Stepanova ² O.I. Lebedev ³ A.V. Zvyagin ¹ S.M. Deyev ⁴ V.Ya. Panchenko ¹

1 FRC - Federal Research Center Krasnoyarsk Scientific Center

2 N.N. Blokhin Russian Cancer Research Center

3 CRISMAT - Laboratoire de cristallographie et sciences des matériaux

4 IBCh RAS - Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry

Abstract : Riboflavin (Rf) is a vitamin and endogenous photosensitizer capable to generate reactive oxygen species (ROS) under UV-blue irradiation and kill cancer cells, which are characterized by the enhanced uptake of Rf. We confirmed its phototoxicity on human breast adenocarcinoma cells SK-BR-3 preincubated with 30-μM Rf and irradiated with ultraviolet light, and proved that such Rf concentrations (60 μM) are attainable in vivo in tumour site by systemic intravascular injection. In order to extend the Rf photosensitization depth in cancer tissue to 6 mm in depth, we purpose-designed core/shell upconversion nanoparticles (UCNPs, NaYF4Yb3+Tm3+/NaYF4) capable to convert 2% of the deeply-penetrating excitation at 975 nm to ultraviolet-blue power. This power was expended to photosensitise Rf and kill SK-BR-3 cells preincubated with UCNPs and Rf, where the UCNP-Rf energy transfer was photon-mediated with ∼14% Förster process contribution. SK-BR-3 xenograft regression in mice was observed for 50 days, following the Rf-UCNPs peritumoural injection and near-infrared light photodynamic treatment of the lesions. © 2016 The Author(s).

Keywords : fluoride metal nanoparticle photosensitizing agent reactive oxygen metabolite sodium yttriumtetrafluoride animal breast tumor C57BL mouse chemistry CHO cell line Cricetulus DBA mouse drug effects drug screening experimental neoplasm human Lewis carcinoma metabolism mouse pathology procedures tumor cell line Animals Breast Neoplasms Carcinoma Lewis Lung Cell Line Tumor Cell Survival CHO Cells Female Fluorides Humans Metal Nanoparticles Mice Inbred C57BL Inbred DBA Neoplasms Experimental Photochemotherapy Photosensitizing Agents Reactive Oxygen Species Riboflavin Thulium Xenograft Model Antitumor Assays Ytterbium Yttrium

Type de document :

Article dans une revue

Domaine :

Chimie

Chimie / Cristallographie

Chimie / Chimie théorique et/ou physique

Chimie / Matériaux

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https://hal-normandie-univ.archives-ouvertes.fr/hal-02184755
Contributeur : Université Normandie <>
Soumis le : mardi 16 juillet 2019 - 11:32:30
Dernière modification le : vendredi 25 octobre 2019 - 10:42:09

Riboflavin photoactivation by upconversion nanoparticles for cancer treatment

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