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Journal articles

Riboflavin photoactivation by upconversion nanoparticles for cancer treatment

E.V. Khaydukov 1 K.E. Mironova 1, 2 V.A. Semchishen 1 A.N. Generalova 1, 2 A.V. Nechaev 1, 3 D.A. Khochenkov 1 E.V. Stepanova 4 O.I. Lebedev 5 A.V. Zvyagin 1, 6 S.M. Deyev 2 V.Ya. Panchenko 1
1 FRC - Federal Research Center Krasnoyarsk Scientific Center
2 IBCh RAS - Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry
3 MSTU Stankin - Moscow State Technological University
4 N.N. Blokhin Russian Cancer Research Center
5 CRISMAT - Laboratoire de cristallographie et sciences des matériaux
6 Macquarie University
Abstract : Riboflavin (Rf) is a vitamin and endogenous photosensitizer capable to generate reactive oxygen species (ROS) under UV-blue irradiation and kill cancer cells, which are characterized by the enhanced uptake of Rf. We confirmed its phototoxicity on human breast adenocarcinoma cells SK-BR-3 preincubated with 30-μM Rf and irradiated with ultraviolet light, and proved that such Rf concentrations (60 μM) are attainable in vivo in tumour site by systemic intravascular injection. In order to extend the Rf photosensitization depth in cancer tissue to 6 mm in depth, we purpose-designed core/shell upconversion nanoparticles (UCNPs, NaYF4Yb3+Tm3+/NaYF4) capable to convert 2% of the deeply-penetrating excitation at 975 nm to ultraviolet-blue power. This power was expended to photosensitise Rf and kill SK-BR-3 cells preincubated with UCNPs and Rf, where the UCNP-Rf energy transfer was photon-mediated with ∼14% Förster process contribution. SK-BR-3 xenograft regression in mice was observed for 50 days, following the Rf-UCNPs peritumoural injection and near-infrared light photodynamic treatment of the lesions. © 2016 The Author(s).
Keywords : CHO Cells Cell Survival Tumor Cell Line Lewis Lung Carcinoma Breast Neoplasms Animals Tumor cell line Procedures Pathology Mouse Metabolism Lewis carcinoma Human Experimental neoplasm Drug screening Drug effects DBA mouse Cricetulus CHO cell line Chemistry C57BL mouse Breast tumor Animal Sodium yttriumtetrafluoride Reactive oxygen metabolite Photosensitizing agent Metal nanoparticle Fluoride Female Fluorides Humans Metal Nanoparticles Mice Inbred C57BL Inbred DBA Neoplasms Experimental Photochemotherapy Photosensitizing Agents Reactive Oxygen Species Riboflavin Thulium Xenograft Model Antitumor Assays Ytterbium Yttrium Biophotonics Drug development
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Submitted on : Tuesday, July 16, 2019 - 11:32:30 AM
Last modification on : Friday, May 6, 2022 - 2:08:39 PM

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COMUE-NORMANDIE | CRISMAT | ENSICAEN | CNRS | UNICAEN | INC-CNRS

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E.V. Khaydukov, K.E. Mironova, V.A. Semchishen, A.N. Generalova, A.V. Nechaev, et al.. Riboflavin photoactivation by upconversion nanoparticles for cancer treatment. Scientific Reports, Nature Publishing Group, 2016, 6, pp.35103. ⟨10.1038/srep35103⟩. ⟨hal-02184755⟩

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