A Genotypic Comparison Reveals That the Improvement in Nitrogen Remobilization Efficiency in Oilseed Rape Leaves Is Related to Specific Patterns of Senescence-Associated Protease Activities and Phytohormones

Marine Poret (1) , Balakumaran Chandrasekar , Renier van Der Hoorn , Sylvain Dechaumet (2) , Alain Bouchereau , Tae-Hwan Kim (3) , Bok-Rye Lee (4) , Flavien Macquart (1) , Ikuko Hara-Nishimura (5) , Jean-Christophe Avice (1)

1 EVA - Ecophysiologie Végétale, Agronomie et Nutritions
2 IGEPP - Institut de Génétique, Environnement et Protection des Plantes
3 Department of economics
4 Department of Metabolic Biology
5 Kyoto University

Oilseed rape ( L.) is an oleoproteaginous crop characterized by low N use efficiency (NUE) that is mainly related to a weak Nitrogen Remobilization Efficiency (NRE) during the sequential leaf senescence of the vegetative stages. Based on the hypothesis that proteolysis efficiency is crucial for the improvement of leafNRE, our objective was to characterize key senescence-associated proteolytic mechanisms of two genotypes (Ténor and Samouraï) previously identified with contrasting NREs. To reach this goal, biochemical changes, protease activities and phytohormone patterns were studied in mature leaves undergoing senescence in two genotypes with contrasting NRE cultivated in a greenhouse under limiting or ample nitrate supply. The genotype with the higher NRE (Ténor) possessed enhanced senescence processes in response to nitrate limitation, and this led to greater degradation of soluble proteins compared to the other genotype (Samouraï). This efficient proteolysis is associated with (i) an increase in serine and cysteine protease (CP) activities and (ii) the appearance of new CP activities (RD21-like, SAG12-like, RD19-like, cathepsin-B, XBCP3-like and aleurain-like proteases) during senescence induced by N limitation. Compared to Samouraï, Ténor has a higher hormonal ratio ([salicylic acid] + [abscisic acid])/([cytokinins]) that promotes senescence, particularly under low N conditions, and this is correlated with the stronger protein degradation and serine/CP activities observed during senescence. Short statement: The improvement in N recycling during leaf senescence in a genotype of L. characterized by a high nitrogen remobilization efficiency is related to a high phytohormonal ratio ([salicylic acid] + [abscisic acid])/([cytokinins]) that promotes leaf senescence and is correlated with an increase or the induction of specific serine and cysteine protease activities.

Mots clés: Brassica napus L.; nitrogen remobilization efficiency; phytohormones; protease activity; regulation; senescence

Domaines: Biologie végétale

Marine Poret, Balakumaran Chandrasekar, Renier van Der Hoorn, Sylvain Dechaumet, Alain Bouchereau, et al.. A Genotypic Comparison Reveals That the Improvement in Nitrogen Remobilization Efficiency in Oilseed Rape Leaves Is Related to Specific Patterns of Senescence-Associated Protease Activities and Phytohormones. Frontiers in Plant Science, 2019, 10, pp.46. ⟨10.3389/fpls.2019.00046⟩. ⟨hal-02184367⟩