Loss of Antibiotic Tolerance in Sod-Deficient Mutants Is Dependent on the Energy Source and Arginine Catabolism in Enterococci

Rabia Ladjouzi; Alain Bizzini; Willem van Schaik; Xinglin Zhang; Alain Rincé; Abdellah Benachour; Axel Hartke; T. Silhavy

Loss of Antibiotic Tolerance in Sod-Deficient Mutants Is Dependent on the Energy Source and Arginine Catabolism in Enterococci

Rabia Ladjouzi ¹ Alain Bizzini ¹ Willem van Schaik ² Xinglin Zhang ² Alain Rincé ¹ Abdellah Benachour ¹ Axel Hartke ¹ T. Silhavy

1 U2RM - Unité de Recherche Risques Microbiens

2 University Medical Center [Utrecht]

Abstract : Enterococci are naturally tolerant to typically bactericidal cell wall-active antibiotics, meaning that their growth is inhibited but they are not killed even when exposed to a high concentration of the drug. The molecular reasons for this extraordinary tolerance are still incompletely understood. Previous work showed that resistance to killing collapsed specifically in mutants affected in superoxide dismutase (Sod) activity, arguing that bactericidal antibiotic treatment led to induction of a superoxide burst. In the present work, we show that loss of antibiotic tolerance in ΔsodA mutants of pathogenic enterococci is dependent on the energy source present during antibiotic treatment. Hexoses induce greater killing than the pentose ribose, and no killing was observed with glycerol as the energy source. These results point to glycolytic reactions as crucial for antibiotic-mediated killing of ΔsodA mutants. A transposon mutant library was constructed in Enterococcus faecalis ΔsodA mutants and screened for restored tolerance of vancomycin. Partially restored tolerance was observed in mutants with transposon integrations into intergenic regions upstream of regulators implicated in arginine catabolism. In these mutants, the arginine deiminase operon was highly upregulated. A model for the action of cell wall-active antibiotics in tolerant and nontolerant bacteria is proposed.

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Article dans une revue

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Sciences du Vivant [q-bio]

Sciences du Vivant [q-bio] / Biochimie, Biologie Moléculaire

Sciences du Vivant [q-bio] / Biochimie, Biologie Moléculaire / Biologie moléculaire

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https://hal-normandie-univ.archives-ouvertes.fr/hal-02180631
Contributeur : Axel Hartke <>
Soumis le : jeudi 11 juillet 2019 - 15:41:59
Dernière modification le : jeudi 29 août 2019 - 01:01:03

Loss of Antibiotic Tolerance in Sod-Deficient Mutants Is Dependent on the Energy Source and Arginine Catabolism in Enterococci

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Loss of Antibiotic Tolerance in Sod-Deficient Mutants Is Dependent on the Energy Source and Arginine Catabolism in Enterococci

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