17β-Estradiol regulates cyclin A1 and cyclin B1 gene expression in adult rat seminiferous tubules

Abstract : Spermatogenesis, which is the fundamental mechanism allowing male gamete production, is controlled by several factors, and among them, estrogens are likely concerned. In order to enlighten the potential role of estrogen in rat spermatogenesis, seminiferous tubules (ST) from two groups of seminiferous epithelium stages (II-VIII and IX-I) were treated with either 17β-estradiol (E(2)) agonists or antagonists for estrogen receptors (ESRs). In this study, we show that cyclin A1 and cyclin B1 gene expression is controlled by E(2) at a concentration of 10(-9) M only in stages IX-I. This effect is mimicked by a treatment with the G-protein coupled estrogen receptor (GPER) agonist G1 and is abolished by treatment with the ESR antagonist ICI 182 780. Moreover, using letrozole, a drug that blocks estrogen synthesis, we demonstrate that these genes are under the control of E(2) within rat ST. Thus, germ cell differentiation may be regulated by E(2) which acts through ESRs and GPER, expressed in adult rat ST.
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https://hal-normandie-univ.archives-ouvertes.fr/hal-02154037
Contributeur : Hélène Bouraïma-Lelong <>
Soumis le : mercredi 12 juin 2019 - 16:19:03
Dernière modification le : samedi 9 novembre 2019 - 01:43:34

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Camille Bois, Christelle Delalande, Hélène Bouraïma-Lelong, Philippe Durand, Serge Carreau. 17β-Estradiol regulates cyclin A1 and cyclin B1 gene expression in adult rat seminiferous tubules. Journal of Molecular Endocrinology, BioScientifica, 2012, 48 (2), pp.89-97. ⟨10.1530/JME-11-0105⟩. ⟨hal-02154037⟩

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