Skip to Main content Skip to Navigation
Journal articles

Acetylcholine Levels and Choline Acetyltransferase Activity in Rat Cerebrovascular Bed after Uni- or Bilateral Sphenopalatine Ganglionectomy

Abstract : Endogenous acetylcholine (ACh) levels and choline acetyltransferase (ChAT) activity were measured in several vascular segments (major cerebral arteries, cortical pial vessels, and peripheral arteries) and nervous tissues [including the sphenopalatine ganglion (SPG)] in the rat. The effects of uni- or bilateral surgical ablation of the SPG, a putative origin of the cholinergic cerebrovascular innervation, were investigated on these two specific cholinergic markers at various postoperative times. ChAT activity and ACh levels were enriched in the cerebral as compared to the peripheral arteries. Among the cerebrovascular tissues tested, ACh levels were particularly high in the circle of Willis and the vertebrobasilar segments and, to a lesser extent, in the middle cerebral artery. Lower levels were found in the small pial vessels and choroid plexus. Overall, ChAT activity measured in different arterial beds paralleled the distribution of ACh. Following uni- or bilateral removal of the SPG, slight reductions (18-36%, statistically not significant) were observed in ChAT activity in rostral cerebral arteries and pial vessels overlying the frontal cortex. Similarly, bilateral ganglionectomy resulted in minor decreases (11-22%, not significant) in the cerebrovascular contents of ACh in these same vascular segments. These results clearly show that the SPG does not or only partly contributes to the cholinergic fibers that supply the cerebrovascular bed.
Complete list of metadatas

https://hal-normandie-univ.archives-ouvertes.fr/hal-02135296
Contributor : François Dauphin <>
Submitted on : Tuesday, May 21, 2019 - 11:00:30 AM
Last modification on : Thursday, June 18, 2020 - 12:32:06 PM

Links full text

Identifiers

Collections

Citation

François Dauphin, Jean W Richard, Jacques Seylaz, Rémi Quirion, Edith Hamel. Acetylcholine Levels and Choline Acetyltransferase Activity in Rat Cerebrovascular Bed after Uni- or Bilateral Sphenopalatine Ganglionectomy. Journal of Cerebral Blood Flow and Metabolism, Nature Publishing Group, 2016, 11 (2), pp.253-260. ⟨10.1038/jcbfm.1991.57⟩. ⟨hal-02135296⟩

Share

Metrics

Record views

43