Transient co-expression for fast and high-yield production of antibodies with human-like N -glycans in plants

Abstract : Plant-based transient expression is potentially the most rapid and cost-efficient system for the production of recombinant pharmaceutical proteins, but safety concerns associated with plant-specific N-glycosylation have hampered its adoption as a commercial production system. In this article, we describe an approach based on the simultaneous transient co-expression of an antibody, a suppressor of silencing and a chimaeric human β 1,4-galactosyltransferase targeted for optimal activity to the early secretory pathway in agroinfiltrated Nicotiana benthamiana leaves. This strategy allows fast and high-yield production of antibodies with human-like N-glycans and, more generally, provides solutions to many critical problems posed by the large-scale production of therapeutic and vaccinal proteins, specifically yield, volume and quality.
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2009 Vezina Plant Biotech J.pd...
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Louis-Philippe Vézina, Loic Faye, Patrice Lerouge, Marc-André D'aoust, Estelle Marquet-Blouin, et al.. Transient co-expression for fast and high-yield production of antibodies with human-like N -glycans in plants. Plant Biotechnology Journal, Wiley, 2009, 7 (5), pp.442-455. ⟨10.1111/j.1467-7652.2009.00414.x⟩. ⟨hal-02128727⟩

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