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Structural characterisation of the pectic polysaccharide rhamnogalacturonan II using an acidic fingerprinting methodology

Martial Seveno 1 Aline Voxeur 1 Christophe Rihouey 2 Ai-Min Wu 3 Tadashi Ishii 4 Christian Chevalier 5 Marie Christine Ralet 6 Azeddine Driouich 1 Alan Marchant 3 Patrice Lerouge 1
1 Glyco-MEV - Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale
2 PBS - Polymères Biopolymères Surfaces
3 University of Southampton
4 FFPRI - Forestry and Forest Products Research Institute
5 BFP - Biologie du fruit et pathologie
6 BIA - Unité de recherche sur les Biopolymères, Interactions Assemblages
Abstract : Rhamnogalacturonan II (RG-II) is a structurally complex cell wall pectic polysaccharide. Despite its complexity, both the structure of RG-II and its ability to dimerise via a borate diester are conserved in vascular plants suggesting that RG-II has a fundamental role in primary cell wall organisation and function. The selection and analysis of new mutants affected in RG-II formation represents a promising strategy to unravel these functions and to identify genes encoding enzymes involved in RG-II biosynthesis. In this paper, a novel fingerprinting strategy is described for the screening of RG-II mutants based on the mild acid hydrolysis of RG-II coupled to the analysis of the resulting fragments by mass spectrometry. This methodology was developed using RG-II fractions isolated from citrus pectins and then validated for RG-II isolated from the Arabidopsis mur1 mutant and irx10 irx10-like double mutant.
Keywords : Cell wall mutants Fingerprinting Mass spectrometry Rhamnogalacturonan II
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Journal articles
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https://hal-normandie-univ.archives-ouvertes.fr/hal-02128719
Contributor : Arnaud Lehner <>
Submitted on : Tuesday, May 14, 2019 - 2:25:09 PM
Last modification on : Tuesday, December 22, 2020 - 12:52:05 PM

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Martial Seveno, Aline Voxeur, Christophe Rihouey, Ai-Min Wu, Tadashi Ishii, et al.. Structural characterisation of the pectic polysaccharide rhamnogalacturonan II using an acidic fingerprinting methodology. Planta, Springer Verlag, 2009, 230 (5), pp.947-957. ⟨10.1007/s00425-009-0996-1⟩. ⟨hal-02128719⟩

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