N-Glycosylation of Plant Recombinant Pharmaceuticals

Muriel Bardor (1) , Gleysin Cabrera (2) , Johannes Stadlmann , Patrice Lerouge (1) , José Cremata (2) , Veronique Gomord (3, 4) , Anne-Catherine Fitchette (3)

1 Glyco-MEV - Laboratoire de Glycobiologie et Matrice Extracellulaire Végétale
2 CIGB - Center for Genetic Engineering and Biotechnology [Cuba]
3 UNIROUEN - Université de Rouen Normandie
4 CNRS - Centre National de la Recherche Scientifique

N-glycosylation is a maturation event necessary for the correct function, efficiency, and stability of a high number of biopharmaceuticals. This chapter presented here proposes various methods to determine whether, how, and where a plant pharmaceutical is N-glycosylated. These methods rely on blot detection with glycan-specific probes, specific deglycosylation of glycoproteins followed by mass spectrometry, N-glycan profile analysis, and glycopeptide identification by LC-MS.

Mots clés: Mass spectrometry; HPLC; N-glycan profiling; MALDI-TOF; LC- MS; Glycosidase; Immunodetection; Western Blotting; Affinodetection; Plant pharmaceuticals; N-glycosylation; Glycopeptide

Domaines: Biologie végétale; Biotechnologies; Organisation et fonctions cellulaires [q-bio.SC]; Génomique, Transcriptomique et Protéomique [q-bio.GN]; Phytopathologie et phytopharmacie; Amélioration des plantes; Biologie moléculaire; Biologie cellulaire; Biochimie [q-bio.BM]; Biologie du développement; Interactions cellulaires [q-bio.CB]

Muriel Bardor, Gleysin Cabrera, Johannes Stadlmann, Patrice Lerouge, José Cremata, et al.. N-Glycosylation of Plant Recombinant Pharmaceuticals. Loïc Faye; Véronique Gomord. Recombinant Proteins From Plants. Methods and Protocols, 483, Springer, pp.239-264, 2009, Methods in Molecular Biology, 978-1-58829-978-9. ⟨10.1007/978-1-59745-407-0_14⟩. ⟨hal-02128578⟩