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Relative Sensitivity Factors of Inorganic Cations in Frozen-hydrated Standards in Secondary Ion MS Analysis

Abstract : We describe the measurement, at 100 K, of the SIMS relative sensitivity factors (RSFs) of the main physiological cations Na+, K+, Mg2+, and Ca2+ in frozen-hydrated (F-H) ionic solutions. Freezing was performed by either plunge freezing or high-pressure freezing. We also report the measurement of the RSFs in flax fibers, which are a model for ions in the plant cell wall, and in F-H ionic samples, which are a model for ions in the vacuole. RSFs were determined under bombardment with neutral oxygen (FAB) for both the fibers and the F-H samples. We show that referencing to ice-characteristic secondary ions is of little value in determining RSFs and that referencing to K is preferable. The RSFs of Na relative to K and of Ca relative to Mg in F-H samples are similar to their respective values in fiber samples, whereas the RSFs of both Ca and Mg relative to K are lower in fibers than in F-H samples. Our data show that the physical factors important for the determination of the RSFs are not the same in F-H samples and in homogeneous matrixes. Our data show that it is possible to perform a SIMS relative quantification of the cations in frozen-hydrated samples with an accuracy on the order of 15%. Referencing to K permits the quantification of the ionic ratios, even when the absolute concentration of the referencing ion is unknown. This is essential for physiological studies of F-H biological samples.
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https://hal-normandie-univ.archives-ouvertes.fr/hal-02115922
Contributor : David Gibouin <>
Submitted on : Tuesday, April 30, 2019 - 3:35:52 PM
Last modification on : Wednesday, August 19, 2020 - 11:17:52 AM

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C. Derue, David Gibouin, F. Lefebvre, D. Studer, M. Thellier, et al.. Relative Sensitivity Factors of Inorganic Cations in Frozen-hydrated Standards in Secondary Ion MS Analysis. Analytical Chemistry, American Chemical Society, 2006, 78 (8), pp.2471-2477. ⟨10.1021/ac051518u⟩. ⟨hal-02115922⟩

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