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A PCR-based method for identification of lactobacilli at the genus level.

Abstract : We developed a polymerase chain reaction (PCR)-based method for the identification of lactobacilli at the genus level. One specific primer, LbLMA1-rev, was designed by analysing similarities between the nucleotide sequence of the spacer between the 16S and 23S rRNA genes in a number of Lactobacillus strains. Amplification with LbLMA1-rev and R16-1, a universal primer, generated a PCR product for 23 Lactobacillus species. Electrophoresis did not reveal any discrete bands when Escherichia coli, Lactococcus lactis, Leuconostoc mesenteroides, Streptococcus thermophilus, Carnobacterium pissicola, Pediococcus pentosaceus, Bifidobacterium bifidum, Weissella confusa, Enterococcus hirae, Staphylococcus aureus or Listeria monocytogenes DNA were used as template.
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https://hal-normandie-univ.archives-ouvertes.fr/hal-02087040
Contributor : Nathalie Desmasures <>
Submitted on : Monday, April 1, 2019 - 5:17:49 PM
Last modification on : Friday, February 14, 2020 - 1:54:02 PM

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Ségolène Dubernet, Nathalie Desmasures, Micheline Gueguen. A PCR-based method for identification of lactobacilli at the genus level.. FEMS Microbiology Letters, Wiley-Blackwell, 2002, 214 (2), pp.271-275. ⟨10.1111/j.1574-6968.2002.tb11358.x⟩. ⟨hal-02087040⟩

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