UGT74B1 from Arabidopsis thaliana as a versatile biocatalyst for the synthesis of desulfoglycosinolates

Sami Marroun (1) , Sabine Montaut , Stéphanie Marquès (2, 3) , Pierre Lafite (3) , Gaël Coadou (1) , Patrick Rollin (4) , Guillaume Jousset (3) , Marie Schuler (3) , Arnaud Tatibouët (3) , Hassan Oulyadi (1) , Richard Daniellou (3)

1 COBRA - Chimie Organique et Bioorganique : Réactivité et Analyse
2 ICBMS - Institut de Chimie et Biochimie Moléculaires et Supramoléculaires
3 ICOA - Institut de Chimie Organique et Analytique
4 ICOA - Institut de Chimie Organique et Analytique

Thioglycosides, even if rare in Nature, have gained increased interest for their biological properties. Chemical syntheses of this class of compounds have been largely studied but little has been reported on their biosynthesis. Herein, combining experiments from the different fields of enzymology, bioorganic chemistry and molecular modeling, we wish to demonstrate the versatility of the glucosyltransferase UGT74B1 and its synthetic potency for the preparation of a variety of natural and unnatural desulfoglycosinolates.

Domains: Chemical Sciences

Sami Marroun, Sabine Montaut, Stéphanie Marquès, Pierre Lafite, Gaël Coadou, et al.. UGT74B1 from Arabidopsis thaliana as a versatile biocatalyst for the synthesis of desulfoglycosinolates. Organic & Biomolecular Chemistry, 2016, 14 (26), pp.6252-6261. ⟨10.1039/c6ob01003b⟩. ⟨hal-02046226⟩