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Journal articles
Readily functionalizable phosphonium-tagged fluorescent coumarins for enhanced detection of conjugates by mass spectrometry
Abstract : Fluorescent coumarins are an important class of small-molecule organic fluorophores ubiquitous in different well-established and emerging fields of research including, among others, biochemistry and chemical biology. The present work aims at covering the poor detectability of coumarin-based conjugates by mass spectrometry while keeping important photophysical properties of the coumarin core. In this context, the synthesis of readily functionalizable phosphonium-tagged coumarin derivatives enabling a dual mass-tag and fluorescence labelling of analytes or (bio)molecules of interest through a single-step protocol, is reported. The utility of these coumarins is illustrated through the preparation of fluorogenic substrates that facilitated identification of the peptide fragment released by specific proteolytic cleavages.
https://hal-normandie-univ.archives-ouvertes.fr/hal-02046223
Contributor : Madeleine Roux-Merlin Connect in order to contact the contributor
Submitted on : Friday, February 22, 2019 - 3:36:58 PM
Last modification on : Wednesday, November 3, 2021 - 5:29:35 AM
Contributor : Madeleine Roux-Merlin Connect in order to contact the contributor
Submitted on : Friday, February 22, 2019 - 3:36:58 PM
Last modification on : Wednesday, November 3, 2021 - 5:29:35 AM
