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Impact of after-treatment devices and biofuels on diesel exhausts genotoxicity in A549 cells exposed at air-liquid interface

Abstract : Using an air-liquid interface (ALI) device in dynamic conditions, we evaluated the efficiency of fuel after-treatment strategies (diesel oxidation catalysis, DOC, and diesel particulate filter, DPF, devices) and the impact of 7% and 30% rapeseed methyl esters (RME) blending on oxidative stress and genotoxicity induced in A549 lung cells after 3 h exposure to whole Diesel exhausts. Oxidative stress was studied using assays of ROS production, glutathione level, catalase and superoxide-dismutase (SOD) activities. No oxidative stress and no clear differences on cytotoxicity patterns between biodiesel and standard Diesel exhausts were found. A weak but significant genotoxicity (8-oxodGuo adducts) and, for standard Diesel only, a DNA damage response (DDR) as evidenced by ƔH2AX foci, remained after DOC + DPF flowing. All together, these data could contribute to the improvement of the after treatment strategies and to health risk assessment of current diesel exhausts.
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https://hal-normandie-univ.archives-ouvertes.fr/hal-02025402
Contributor : François Sichel <>
Submitted on : Tuesday, February 19, 2019 - 4:22:35 PM
Last modification on : Monday, July 6, 2020 - 11:24:02 AM

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Claire Barraud, Cécile Corbière, I. Pottier, E. Estace, K. Blanchard, et al.. Impact of after-treatment devices and biofuels on diesel exhausts genotoxicity in A549 cells exposed at air-liquid interface. Toxicology in Vitro, Elsevier, 2017, 45 (Part 3), pp.426-433. ⟨10.1016/j.tiv.2017.04.025⟩. ⟨hal-02025402⟩

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