Isolation and Structural Characterization of Two Novel Peptides Derived from Proopiomelanocortin in the Pituitary of the Rainbow Trout

Hélène Tollemer (1, 2) , Jérôme Leprince (1, 2) , Ludovic Galas (1, 2) , Frans Vandesande (3) , Jean-Claude Le Mevel (4) , Marie-Christine Tonon (1, 2) , J.Michael Conlon (5) , Hubert Vaudry (1, 2)

1 Neuroendocrinologie cellulaire et moléculaire
2 IFRMP 23 - Institut Fédératif de Recherches Multidisciplinaires sur les Peptides
3 KU Leuven - Catholic University of Leuven = Katholieke Universiteit Leuven
4 UBO UFR MSS - Université de Bretagne Occidentale - UFR Médecine et Sciences de la Santé
5 Creighton University Medical School [Omaha, NE, USA]

The trout possesses two POMC genes as a result of duplication of its genome some 25-100 million years ago. One of the POMC molecules exhibits a unique C-terminal extension of 25 amino acid residues which is not found in any other POMC characterized so far. In order to isolate possible novel peptides derived from trout POMC-A, we have raised antibodies against two synthetic epitopes derived from the C-terminal region of the precursor. Two native decapeptides were isolated in pure form from an extract of trout pituitary. The primary structures of these peptides were established as Glu-Gln-Trp-Gly-Arg-Glu-Glu-Gly-Glu-Glu and Ala-Leu-Gly-Glu-Arg-Lys-Tyr-His-Phe-Gln-NH2. The structure of the trout POMC-A cDNA reveals that both peptides are flanked by pairs of basic amino acids or a glycine residue, indicating that they can actually be generated during post-translational processing of POMC-A.

Domaines: Chimie thérapeutique; Interactions cellulaires [q-bio.CB]; Neurobiologie; Pharmacologie

Hélène Tollemer, Jérôme Leprince, Ludovic Galas, Frans Vandesande, Jean-Claude Le Mevel, et al.. Isolation and Structural Characterization of Two Novel Peptides Derived from Proopiomelanocortin in the Pituitary of the Rainbow Trout. Biochemical and Biophysical Research Communications, 1997, 238 (2), pp.653-657. ⟨10.1006/bbrc.1997.7356⟩. ⟨hal-01974282⟩